ArticleJournal of clinical microbiology2007
Characterization of in vitro biofilm-associated pneumococcal phase variants of a clinically relevant serotype 3 clone.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- Article
- Subversion of host immune responses by otopathogens during otitis media.Journal of leukocyte biology · 2019Review
- DropSOAC: Stabilizing Microfluidic Drops for Time-Lapse Quantification of Single-Cell Bacterial Physiology.Frontiers in microbiology · 2019Article
- Phase Variation ofMicrobiology spectrum · 2019Article
- Rutin InhibitsFrontiers in pharmacology · 2017Article
- New Technologies for Studying Biofilms.Microbiology spectrum · 2015Review
- A point mutation in cpsE renders Streptococcus pneumoniae nonencapsulated and enhances its growth, adherence and competence.BMC microbiology · 2014Article
- Biofilm formation in Streptococcus pneumoniae.Microbial biotechnology · 2012Review
- Heterogeneity in Pseudomonas aeruginosa biofilms includes expression of ribosome hibernation factors in the antibiotic-tolerant subpopulation and hypoxia-induced stress response in the metabolically active population.Journal of bacteriology · 2012Article
- The impact of the competence quorum sensing system on Streptococcus pneumoniae biofilms varies depending on the experimental model.BMC microbiology · 2011Article
- Signal diffusion and the mitigation of social exploitation in pneumococcal competence signalling.Proceedings. Biological sciences · 2010Article
- Coinfection with Haemophilus influenzae promotes pneumococcal biofilm formation during experimental otitis media and impedes the progression of pneumococcal disease.The Journal of infectious diseases · 2010Article
- Simultaneous nasopharyngeal carriage of two pneumococcal multilocus sequence types with a serotype 3 phenotype.International journal of microbiology · 2010Article
- Isolation of Streptococcus pneumoniae biofilm mutants and their characterization during nasopharyngeal colonization.Infection and immunity · 2008Article
- Characterization of biofilm matrix, degradation by DNase treatment and evidence of capsule downregulation in Streptococcus pneumoniae clinical isolates.BMC microbiology · 2008Article
- Formation of Streptococcus pneumoniae non-phase-variable colony variants is due to increased mutation frequency present under biofilm growth conditions.Journal of bacteriology · 2008Article
- Characterization of colony morphology variants isolated from Streptococcus pneumoniae biofilms.Journal of bacteriology · 2007Article
- Early biofilm formation on microtiter plates is not correlated with the invasive disease potential of Streptococcus pneumoniae.Microbial pathogenesisArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
An increasing proportion of children with acute otitis media due to Streptococcus pneumoniae have serotype 3 infections since licensure of the seven-valent pneumococcal conjugate vaccine. These serotype 3 strains are genetically related by molecular subtyping. During otitis media with effusion and recurrent otitis media, biofilms commonly develop. Pneumococcal in vitro biofilms are comprised of phase variants that differ in colony morphology. By using a representative strain of the mucoid serotype 3 clone, rough phase variants with a diverse array of mutations were detected in biofilms formed in vitro. Most phase variants had mutations in the cps3D gene, the first gene of the capsular operon. Eleven had single nucleotide polymorphisms (SNPs) in the cps3D gene, one had an SNP in the -10 promoter, and three had large deletions in the cps3D gene. Reversion to the mucoid phenotype was associated with reversion of the mutation in the cps3D gene. Unlike the phase variants detected in the nasopharynx, which have at least 20% of the parental amount of capsule, the in vitro biofilm-associated phase variants had < or =12% of the parental amount of capsule, as determined by capsule enzyme-linked immunosorbent assays. Using real-time reverse transcription-PCR, we determined that capsule expression in the phase variants was likely regulated at multiple levels. These in vitro phase variation data, which underscore the plasticity of the pneumococcus, need to be confirmed with in vivo analyses of the middle ear mucosa during otitis media.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.