ArticleJournal of clinical microbiology2007
Validation of pulsed-field gel electrophoresis and spa typing for long-term, nationwide epidemiological surveillance studies of Staphylococcus aureus infections.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
60 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Methicillin-ResistantToxins · 2020Pooled it
- Preventing surgical site infections: a randomized, open-label trial of nasal mupirocin ointment and nasal povidone-iodine solution.Infection control and hospital epidemiology · 2014Trial
- Genetic Diversity and Epidemiological Overlap of Staphylococcus aureus at the Animal-Food-Environment-Human Interface Within a One Health Framework.MicrobiologyOpen · 2026Article
- Multidrug resistance including borderline-oxacillin resistance and spa typing of Staphylococcus aureus from milk and milk products of Tigray region, Northern Ethiopia.BMC veterinary research · 2025Article
- Emergence and spread of a mupirocin-resistant variant of the European epidemic fusidic acid-resistant impetigo clone ofEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2024Article
- Persistence of the Staphylococcus aureus epidemic European fusidic acid-resistant impetigo clone (EEFIC) in Belgium.The Journal of antimicrobial chemotherapy · 2023Article
- Antibiotic Resistance Diagnosis in ESKAPE Pathogens-A Review on Proteomic Perspective.Diagnostics (Basel, Switzerland) · 2023Review
- Antimicrobial Resistance, SCCAntibiotics (Basel, Switzerland) · 2023Article
- Antimicrobial resistance studies in staphylococci and streptococci isolated from cows with mastitis in Argentina.Journal of veterinary science · 2022Article
- Antimicrobial Resistance and Molecular Characterization ofAntibiotics (Basel, Switzerland) · 2022Article
- Diversity of SCCmec elements and spa types in South African Staphylococcus aureus mecA-positive blood culture isolates.BMC infectious diseases · 2020Article
- Control of Methicillin-ResistantFrontiers in microbiology · 2020Article
- Methicillin-Resistant Staphylococcus aureus: Molecular Characterization, Evolution, and Epidemiology.Clinical microbiology reviews · 2018Review
- Article
- Article
- Typing of Methicillin Resistant Staphylococcus Aureus Using DNA Fingerprints by Pulsed-field Gel Electrophoresis.Acta informatica medica : AIM : journal of the Society for Medical Informatics of Bosnia & Herzegovina : casopis Drustva za medicinsku informatiku BiH · 2016Article
- Spa Typing of Staphylococcus aureus Strains Isolated From Clinical Specimens of Patients With Nosocomial Infections in Tehran, Iran.Jundishapur journal of microbiology · 2016Article
- In vitro selection of Staphylococcus aureus mutants resistant to tigecycline with intermediate susceptibility to vancomycin.Annals of clinical microbiology and antimicrobials · 2016Article
- Identifying the effect of patient sharing on between-hospital genetic differentiation of methicillin-resistant Staphylococcus aureus.Genome medicine · 2016Article
- A 3-year long study of Staphylococcus aureus isolates from subclinical mastitis in three Azawak zebu herds at the Sahelian experimental farm of Toukounous, Niger.Tropical animal health and production · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pulsed-field gel electrophoresis (PFGE) of genomic macrorestriction fragments has been used by the Belgian Reference Laboratory for Staphylococci for national hospital surveys of methicillin-resistant Staphylococcus aureus since 1992. The sequencing of the polymorphic X region of the protein A gene (spa typing) offers significant advantages over PFGE in terms of speed, ease of interpretation, and exportability. To validate its potential use for national surveillance, we evaluated the robustness of spa typing compared with that of PFGE based on a collection of 217 S. aureus strains representative of the Belgian S. aureus epidemiology during the last 13 years. spa typing and PFGE both showed high discriminatory power (discriminatory indexes of 0.98 and 0.96, respectively) and achieved high concordance (95.9%) in type classification. Both methods also showed good concordance with multilocus sequence typing (MLST) (95.5%). However, we observed occasional "violations" of MLST clonal complex assignment by spa typing. Our results suggest that both PFGE and spa typing are reliable methods for long-term, nationwide epidemiological surveillance studies. We suggest that spa typing, which is a single-locus-based method, should preferably be used in combination with additional markers, such as staphylococcal cassette chromosome mec typing or resistance or virulence gene detection.
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