ArticleJournal of clinical microbiology2007
Rapid detection and identification of metallo-beta-lactamase-encoding genes by multiplex real-time PCR assay and melt curve analysis.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 117 papers.
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Who cites it
117 citing papers in PubMed.
- Multiplex real-time PCR with high-resolution melting analysis for rapid identification of carbapenem and colistin resistance genes in clinical Enterobacterales isolates.Scientific reports · 2026Article
- Wild Fishes as Reservoirs of Gut Bacteria Carrying Antimicrobial Resistance Encoding Genes in Chilean Bays.Antibiotics (Basel, Switzerland) · 2026Article
- Environmental reservoirs account for high levels of carbapenem resistance genes in wastewater.Microbiology spectrum · 2026Article
- Impact of the COVID-19 Pandemic on Carbapenem-ResistantAntibiotics (Basel, Switzerland) · 2025Article
- Development and Validation of a Multiplex Real-Time PCR Assay for Rapid Screening of Main Carbapenemase Genes in Clinical Isolates and Surveillance Samples.Antibiotics (Basel, Switzerland) · 2025Article
- Phenotypic and Molecular Characterization of Extended-Spectrum β-Lactamase, Plasmid-Mediated- AmpC, and Carbapenemase-Producing Enterobacteriaceae Isolated from Companion and Production Animals in Brazil.Current microbiology · 2025Article
- Impact of ExogenousAntibiotics (Basel, Switzerland) · 2024Article
- Genomic Characterization of 16S rRNA Methyltransferase-ProducingAntibiotics (Basel, Switzerland) · 2024Article
- Clonal Distribution and Its Association With the Carbapenem Resistance Mechanisms of Carbapenem-Non-SusceptibleAnnals of laboratory medicine · 2024Article
- Development of a Simple Method to Detect the Carbapenemase-Producing GenesDiagnostics (Basel, Switzerland) · 2024Article
- Genomic Characterization of Carbapenemase-ProducingAntibiotics (Basel, Switzerland) · 2024Article
- Analyses of Extended-Spectrum-β-Lactamase, Metallo-β-Lactamase, and AmpC-β-Lactamase Producing Enterobacteriaceae from the Dairy Value Chain in India.Antibiotics (Basel, Switzerland) · 2023Article
- Genomic Characterization of IMP-ProducingMicroorganisms · 2023Article
- Endemic High-Risk Clone ST277 Is Related to the Spread of SPM-1-ProducingMicroorganisms · 2023Article
- Nationwide, population-based observational study of the molecular epidemiology and temporal trend of carbapenemase-producing Enterobacterales in Norway, 2015 to 2021.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2023Observational
- Screening and validation of salt-stress responsive cg-SSR markers in wheat (Triticum aestivum L.) germplasm of Pakistan.Molecular biology reports · 2023Article
- Genotypic Determination of Extended Spectrum β-Lactamases and Carbapenemase Production in Clinical Isolates ofInfectious disease reports · 2023Article
- Early appropriate diagnostics and treatment of MDR Gram-negative infections.JAC-antimicrobial resistance · 2022Review
- Emerging Diabetic Foot Ulcer Microbiome Analysis Using Cutting Edge Technologies.Journal of diabetes science and technology · 2022Article
- Clinical outcome from hematopoietic cell transplant patients with bloodstream infection caused by carbapenem-resistant P. aeruginosa and the impact of antimicrobial combination in vitro.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2022Article
57 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metallo-beta-lactamase enzymes (MbetaL) are encoded by transferable genes, which appear to spread rapidly among gram-negative bacteria. The objective of this study was to develop a multiplex real-time PCR assay followed by a melt curve step for rapid detection and identification of genes encoding MbetaL-type enzymes based on the amplicon melting peak. The reference sequences of all genes encoding IMP and VIM types, SPM-1, GIM-1, and SIM-1 were downloaded from GenBank, and primers were designed to obtain amplicons showing different sizes and melting peak temperatures (Tm). The real-time PCR assay was able to detect all MbetaL-harboring clinical isolates, and the Tm-assigned genotypes were 100% coincident with previous sequencing results. This assay could be suitable for identification of MbetaL-producing gram-negative bacteria by molecular diagnostic laboratories.
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