ArticleJournal of clinical microbiology2007
Clonal complexes of Campylobacter jejuni identified by multilocus sequence typing are reliably predicted by restriction fragment length polymorphism analyses of the flaA gene.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- Campycins are novel broad-spectrum antibacterials killing Campylobacter jejuni.Applied microbiology and biotechnology · 2024Article
- Genomic diversity of Campylobacter jejuni and Campylobacter coli isolated from the Ethiopian dairy supply chain.PloS one · 2024Article
- Investigation of MALDI-TOF Mass Spectrometry for Assessing the Molecular Diversity ofDiagnostics (Basel, Switzerland) · 2021Article
- Developing Innolysins AgainstFrontiers in microbiology · 2021Article
- Article
- Critical Orientation in the Jungle of Currently Available Methods and Types of Data for Source Attribution of Foodborne Diseases.Frontiers in microbiology · 2019Review
- Prevalence, antimicrobial resistance and genetic diversity of Campylobacter coli and Campylobacter jejuni in Ecuadorian broilers at slaughter age.Poultry science · 2017Article
- Multilocus sequence typing and FlaA sequencing reveal the genetic stability of Campylobacter jejuni enrichment during coculture with Acanthamoeba polyphaga.Applied and environmental microbiology · 2013Article
- Phenotypic and genotypic characterizations of Campylobacter jejuni isolated from the broiler meat production process.Current microbiology · 2012Article
- Comprehensive detection and discrimination of Campylobacter species by use of confocal micro-Raman spectroscopy and multilocus sequence typing.Journal of clinical microbiology · 2012Article
- Development and validation of a comparative genomic fingerprinting method for high-resolution genotyping of Campylobacter jejuni.Journal of clinical microbiology · 2012Article
- Comparison of molecular typing methods useful for detecting clusters of Campylobacter jejuni and C. coli isolates through routine surveillance.Journal of clinical microbiology · 2012Article
- High-resolution melting system to perform multilocus sequence typing of Campylobacter jejuni.PloS one · 2011Article
- Scientific Opinion on Quantification of the risk posed by broiler meat to human campylobacteriosis in the EU.EFSA journal. European Food Safety Authority · 2010Article
- Use of pcr-rflp of the fla a gene for detection and subtyping of Campylobacter jejuni strains Potentially related to Guillain-barré syndrome, isolated from humans and animals.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2009Article
- Risk factors for infection with Campylobacter jejuni flaA genotypes.Epidemiology and infection · 2008Article
- Multilocus sequence typing of Campylobacter jejuni isolates from humans, chickens, raw milk, and environmental water in Quebec, Canada.Journal of clinical microbiology · 2008Article
- Epidemiology, relative invasive ability, molecular characterization, and competitive performance of Campylobacter jejuni strains in the chicken gut.Applied and environmental microbiology · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multilocus sequence typing (MLST) has provided important new insights into the population structure of Campylobacter jejuni and is rapidly becoming the gold standard for typing this species. However, the methodology is comparatively costly and slow to perform for the routine surveillance testing of large numbers of isolates required by public health laboratories. Restriction fragment length polymorphism analysis of the flaA gene (RFLP-flaA) and sequencing of the variable region in the fla locus (SVR-fla) were compared to MLST to determine if a low cost alternative could be found that reliably predicts clonal lineage (as determined by MLST). An isolate of C. jejuni from each of 153 patients from New South Wales, Australia, collected sequentially over a period of 30 months from 1999 to 2001 and comprising 40 sequence types (ST) from 15 clonal complexes (CC) was examined. Of 15 CC, 12 were represented by more than one isolate and a predominant RFLP-flaA type was found for 10 (83%). Of these, seven (70%) correctly predicted the predominant MLST CC with a probability of >0.8. Of 40 STs detected, 19 were reported for the first time, 9 of which were represented by more than one isolate. Eight of these were represented by a single RFLP-flaA type. Only two of eight major SVR-fla types were able to predict CC with a probability of >0.8, indicating that flaA-RFLP is a more reliable predictor of CC than SVR-fla and thus offers an alternative to MLST for use in routine surveillance.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.