Evidence map›Paper›PMID 17092001›Full record

ArticleThe journal of physical chemistry. B2006

Enhanced fluorescence of Cy5-labeled oligonucleotides near silver island films: a distance effect study using single molecule spectroscopy.

Yi Fu, Joseph R Lakowicz

Abstract read
In one paragraph

Article in The journal of physical chemistry. B, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
3.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 59 citations in OpenAlex.

  1. Chemiplasmonics for high-throughput biosensors.International journal of nanomedicine · 2018
    Article
  2. Article
  3. Review
  4. Article
  5. Enhanced Single-Molecule Detection using Porous Silver Membrane.The journal of physical chemistry. C, Nanomaterials and interfaces · 2010
    Article
  6. Article
  7. Article
  8. Plasmon-controlled fluorescence towards high-sensitivity optical sensing.Advances in biochemical engineering/biotechnology · 2009
    Review
  9. Metal-enhanced fluorescence of single green fluorescent protein (GFP).Biochemical and biophysical research communications · 2008
    Article
  10. Article
  11. Review
  12. Article
  13. Article
  14. Single molecule photophysics near metallic nanostructures.Proceedings of SPIE--the International Society for Optical Engineering · 2008
    Article
  15. Single-Molecule Studies on Fluorescently Labeled Silver Particles: Effects of Particle Size.The journal of physical chemistry. C, Nanomaterials and interfaces · 2007
    Article
  16. Metal-Enhanced Fluorescence of Phycobiliproteins from Heterogeneous Plasmonic Nanostructures.The journal of physical chemistry. C, Nanomaterials and interfaces · 2007
    Article
  17. Fluorescence images of DNA-bound YOYO between coupled silver particles.Langmuir : the ACS journal of surfaces and colloids · 2007
    Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Yi FuCenter for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, Baltimore, Maryland 21201 USA. lakowicz@cfs.umbi.umd.edu
Joseph R Lakowicz
University of Maryland, Baltimore · US

Funding

WHO'S WHO IN FLUORESCENCEP41RR008119 · NCRR · UNIVERSITY OF MARYLAND BALTIMORE · PI BASKAKOV, ILIA V · 1993 to 2006
$7.4M
Metal-Enhanced Fluorescence SensingR01EB000682 · NIBIB · UNIVERSITY OF MARYLAND BALTIMORE · PI LAKOWICZ, JOSEPH R. · 2002 to 2006
$1.7M
NCRR NIH HHS P41 RR008119NCRR NIH HHS RR-08119NIBIB NIH HHS EB-00682NIBIB NIH HHS R01 EB000682
6 · The paper itself

Abstract

We investigated fluorescence enhancements and lifetime reductions of Cy5 probe molecules at various distances from the deposited silver island film surface using single molecule spectroscopic methods. The proximity of fluorophore molecules to the surface was controlled by alternating layers of biotinylated bovine serum albumin (BSA-biotin) and avidin, followed by binding of Cy5-labeled oligonucleotides to the top of a BSA-biotin layer structure. We observed dramatically varied brightness of fluorophores with distances from metal structures as well with reduced blinking in the presence of silver island films. In addition, distributions of fluorescence lifetimes and apparent emission intensities from individual molecules indicate an inhomogeneous nature of local matrix surface near metallic nanostructures. These studies illustrate the exclusive information that is otherwise hidden in ensemble measurements.

Indexed as

Binding SitesBiotinylationCarbocyaninesFluorescent DyesOligonucleotidesSerum Albumin, BovineSilverSpectrometry, FluorescenceTime FactorsCarbocyaninescyanine dye 5Fluorescent DyesOligonucleotidesSerum Albumin, BovineSilver

Identifiers

PMID17092001
PMCPMC2737407
OpenAlexW1979160748

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.