ArticleThe Journal of cell biology1991
Keratin incorporation into intermediate filament networks is a rapid process.
Article in The Journal of cell biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 94 citations in OpenAlex.
- Structural heterogeneity of cellular K5/K14 filaments as revealed by cryo-electron microscopy.eLife · 2021Article
- Types I and II Keratin Intermediate Filaments.Cold Spring Harbor perspectives in biology · 2018Review
- Site-specific glycosylation regulates the form and function of the intermediate filament cytoskeleton.eLife · 2018Article
- Intermediate Filaments at the Junction of Mechanotransduction, Migration, and Development.Frontiers in cell and developmental biology · 2017Review
- Emerging roles of sumoylation in the regulation of actin, microtubules, intermediate filaments, and septins.Cytoskeleton (Hoboken, N.J.) · 2015Review
- Intermediate filaments: a dynamic network that controls cell mechanics.F1000prime reports · 2014Review
- Measuring the regulation of keratin filament network dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Inroads into the structure and function of intermediate filament networks.Journal of structural biology · 2012Review
- Cytoskeleton in motion: the dynamics of keratin intermediate filaments in epithelia.The Journal of cell biology · 2011Review
- "Panta rhei": Perpetual cycling of the keratin cytoskeleton.Bioarchitecture · 2011Article
- Article
- Introducing intermediate filaments: from discovery to disease.The Journal of clinical investigation · 2009Review
- Insights into the mechanical properties of epithelial cells: the effects of shear stress on the assembly and remodeling of keratin intermediate filaments.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2009Article
- Bacterial intermediate filaments: in vivo assembly, organization, and dynamics of crescentin.Genes & development · 2009Article
- Mechanical strain of alveolar type II cells in culture: changes in the transcellular cytokeratin network and adaptations.American journal of physiology. Lung cellular and molecular physiology · 2008Article
- Intermediate filaments in smooth muscle.American journal of physiology. Cell physiology · 2008Review
- Intermediate filaments: versatile building blocks of cell structure.Current opinion in cell biology · 2008Review
- Identification of novel principles of keratin filament network turnover in living cells.Molecular biology of the cell · 2004Article
- Insights into the dynamic properties of keratin intermediate filaments in living epithelial cells.The Journal of cell biology · 2001Article
- Desmin filaments studied by quasi-elastic light scattering.Biophysical journal · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The properties of keratin-containing intermediate filament (IF) networks in vivo were studied following the microinjection of biotinylated keratin. Keratin-IFs were biotinylated, disassembled, and separated into type I and type II proteins by ion exchange chromatography. Recombination of these derivatized type I and type II keratins resulted in the formation of 10-nm diameter IF. The type I keratins were microinjected into epithelial cells and observed by immunofluorescence microscopy. Biotin-rich spots were found throughout the cytoplasm at 15-20 min after injection. Short biotinylated fibrous structures were seen at 30-45 min after injection, most of which colocalized with the endogenous bundles of IF (tono-filaments). By 1 1/2 to 2 h after microinjection, extensive biotinylated keratin IF-like networks were evident. These were highly coincident with the endogenous tonofilaments throughout the cell, including those at desmosomal junctions. These results suggest the existence of a relatively rapid subunit incorporation mechanism using numerous sites along the length of the endogenous tonofilament bundles. These observations support the idea that keratin-IFs are dynamic cytoskeletal elements.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.