ArticleMammalian genome : official journal of the International Mammalian Genome Society2006
Cloning of the bovine prion-like Shadoo (SPRN) gene by comparative analysis of the predicted genomic locus.
Article in Mammalian genome : official journal of the International Mammalian Genome Society, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 20 citations in OpenAlex.
- Scrapie susceptibility-associated indel polymorphism of shadow of prion protein gene (SPRN) in Korean native black goats.Scientific reports · 2019Article
- Genetics of Prion Disease in Cattle.Bioinformatics and biology insights · 2015Review
- Cellular prion protein: from physiology to pathology.Viruses · 2012Review
- PRNP and SPRN genes polymorphism in atypical bovine spongiform encephalopathy cases diagnosed in Polish cattle.Journal of applied genetics · 2012Article
- Characterization of PRNP and SPRN coding regions from atypical scrapie cases diagnosed in Poland.Molecular biology reports · 2012Article
- Comparative analysis of the Shadoo gene between cattle and buffalo reveals significant differences.PloS one · 2012Article
- Identification of the RGG box motif in Shadoo: RNA-binding and signaling roles?Bioinformatics and biology insights · 2008Article
- Characterization of the genomic region containing the Shadow of Prion Protein (SPRN) gene in sheep.BMC genomics · 2007Article
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Authors and funding
11 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
SPRN is a new prion-like gene coding for Sho, a protein with significant similarity to PrP. SPRN was initially described in zebrafish; however, the strong evolutionary conservation led to the hypothesis that SPRN might be the ancestral prion-like gene. We mapped SPRN in Bos taurus by comparative analysis of the locus and of the predicted flanking genes. BACs, spanning the whole SPRN genomic locus, were assigned to BTA26q23 by radiation hybrid mapping and fluorescent in situ hybridization (FISH). Sequencing of five genes flanking SPRN, namely, ECHS1, PAOX, MTG1, SPRN, and CYP2E1, high-resolution FISH on mechanically stretched chromosomes, and combed BAC DNA allowed us to establish their order and reciprocal orientation. The results confirmed that BTA26q23 corresponds to HSA10q24.3-26.3, which is the site where the human SPRN is located. The gene order in Bos taurus is the same as in man, cen-ECHS1-PAOX-MTG1-SPRN-CYP2E1-tel, but PAOX has a different orientation in the two species. SPRN has the typical two-exon PRNP arrangement, with the CDS fully contained within exon 2; furthermore, it codes for a 143-amino-acid protein with 74.8% identity and 84.7% similarity with the human PRNP. RT-PCR and Northern blot analysis showed that SPRN is expressed at high levels in brain and less in testis and lung.
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