ArticleNucleic acids research2006
The mechanism of gene targeting in Physcomitrella patens: homologous recombination, concatenation and multiple integration.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed.
- The Importance of thePlants (Basel, Switzerland) · 2026Review
- tRNA-based polycistronic CRISPR/Cas9 system boosts efficiency of multi-gene deletion in the moss Physcomitrella.Plant methods · 2026Article
- Functional characterisation of Target of Rapamycin (TOR) signalling in Physcomitrella.Plant cell reports · 2026Article
- Physcomitrium patens: an emerging model for autophagy study.Protoplasma · 2025Review
- TRB proteins in moss reveal their evolutionarily conserved roles in plant development and telomere maintenance.The Plant journal : for cell and molecular biology · 2025Article
- Flavodiiron proteins in Physcomitrium patens: navigating the edge between photoprotection and efficiency.The Plant journal : for cell and molecular biology · 2025Article
- Designing a synthetic moss genome using GenoDesigner.Nature plants · 2024Review
- Telomere Length Variation in Model Bryophytes.Plants (Basel, Switzerland) · 2024Article
- A cornichon protein controls polar localization of the PINA auxin transporter in Physcomitrium patens.Development (Cambridge, England) · 2023Article
- A deeply conserved protease, acylamino acid-releasing enzyme (AARE), acts in ageing in Physcomitrella and Arabidopsis.Communications biology · 2023Article
- Autopolyploidization affects transcript patterns and gene targeting frequencies in Physcomitrella.Plant cell reports · 2022Article
- SOG1, a plant-specific master regulator of DNA damage responses, originated from nonvascular land plants.Plant direct · 2021Article
- Targeted Gene Knockouts by Protoplast Transformation in the MossFrontiers in genome editing · 2021Review
- Genome-wide analysis of LATERAL ORGAN BOUNDARIES DOMAIN-in Physcomitrella patens and stress responses.Genes & genomics · 2020Article
- Transient cotransformation of CRISPR/Cas9 and oligonucleotide templates enables efficient editing of target loci in Physcomitrella patens.Plant biotechnology journal · 2020Article
- Cytokinin oxidase PpCKX1 plays regulatory roles in development and enhances dehydration and salt tolerance in Physcomitrella patens.Plant cell reports · 2020Article
- Stable Protein Sialylation in Physcomitrella.Frontiers in plant science · 2020Article
- A Chloroplast COR413 Protein FromFrontiers in plant science · 2020Article
- The AVR4 effector is involved in cercosporin biosynthesis and likely affects the virulence of Cercospora cf. flagellaris on soybean.Molecular plant pathology · 2020Article
- Regeneration and transient gene expression in protoplasts ofPlant methods · 2019Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The model bryophyte Physcomitrella patens exhibits high frequencies of gene targeting when transformed with DNA constructs containing sequences homologous with genomic loci. 'Targeted gene replacement' (TGR) resulting from homologous recombination (HR) between each end of a targeting construct and the targeted locus occurs when either single or multiple targeting vectors are delivered. In the latter instance simultaneous, multiple, independent integration of different transgenes occurs at the targeted loci. In both single gene and 'batch' transformations, DNA can also be found to undergo 'targeted insertion' (TI), integrating at one end of the targeted locus by HR with one flanking sequence of the vector accompanied by an apparent non-homologous end-joining (NHEJ) event at the other. Untargeted integration at nonhomologous sites also occurs, but at a lower frequency. Molecular analysis of TI at a single locus shows that this occurs as a consequence of concatenation of the transforming DNA, in planta, prior to integration, followed by HR between a single site in the genomic target and two of its repeated homologues in the concatenated vector. This reinforces the view that HR is the major pathway by which transforming DNA is integrated in Physcomitrella.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.