ArticleMolecular genetics and genomics : MGG2007
Stress-inducible DREB2A transcription factor from Pennisetum glaucum is a phosphoprotein and its phosphorylation negatively regulates its DNA-binding activity.
Article in Molecular genetics and genomics : MGG, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- Identification of DREB gene family in foxtail millet (Frontiers in plant science · 2025Article
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- Comparative proteomics in tall fescue to reveal underlying mechanisms for improving Photosystem II thermotolerance during heat stress memory.BMC genomics · 2024Article
- Genome-Wide Identification and Transcriptional Analysis ofInternational journal of molecular sciences · 2024Article
- A pervasive phosphorylation cascade modulation of plant transcription factors in response to abiotic stress.Planta · 2023Review
- Expression of a Pennisetum glaucum gene DREB2A confers enhanced heat, drought and salinity tolerance in transgenic Arabidopsis.Molecular biology reports · 2022Article
- Article
- Fine-tuning of SUMOylation modulates drought tolerance of apple.Plant biotechnology journal · 2022Article
- Current perspectives of ubiquitination and SUMOylation in abiotic stress tolerance in plants.Frontiers in plant science · 2022Review
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- Applications of CRISPR/Cas9 as New Strategies for Short Breeding to Drought Gene in Rice.Frontiers in plant science · 2022Article
- Growth responses and differential expression ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
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- Understanding the Integrated Pathways and Mechanisms of Transporters, Protein Kinases, and Transcription Factors in Plants under Salt Stress.International journal of genomics · 2021Review
- Genome-Wide Analysis of the C2 Domain Family in Soybean and Identification of a Putative Abiotic Stress Response GeneFrontiers in plant science · 2021Article
- Characterization of novel regulators for heat stress tolerance in tomato from Indian sub-continent.Plant biotechnology journal · 2020Article
- Article
- Computational Disorder Analysis in Ethylene Response Factors Uncovers Binding Motifs Critical to Their Diverse Functions.International journal of molecular sciences · 2019Article
- An Arabidopsis E3 ligase HUB2 increases histone H2B monoubiquitination and enhances drought tolerance in transgenic cotton.Plant biotechnology journal · 2019Article
- Article
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5 authors.
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Abstract
Abiotic stress-mediated gene expression is regulated via different transcription factors of which drought-responsive element-binding (DREB) proteins play an important role. There are two types of DREBs. Presently, the function of DREB1 type protein is well studied; however, much less information is available for DREB2. In this study, a cDNA with an open reading frame of 332 amino acids, encoding the transcription activation factor DREB2A, was cloned from Pennisetum glaucum, a stress tolerant food grain crop. Phylogenetic tree revealed that PgDREB2A is more close to DREBs isolated from monocots, though it forms an independent branch. The PgDREB2A transcript was up-regulated in response to drought within 1 h of the treatment, whereas the induction was delayed in response to cold and salinity stress. However, during cold stress, the transcript was induced more as compared to drought and salinity. The recombinant PgDREB2A protein having a molecular mass of 36.6 kDa was purified using Ni-NTA affinity chromatography. Gel mobility shift assays using the purified protein and two cis elements of rd29A (responsive to dehydration 29A) gene promoter of Arabidopsis revealed that PgDREB2A binds to drought-responsive element (DRE) ACCGAC and not to GCCGAC. PgDREB2A is a phosphoprotein, which has not been reported earlier. The phosphorylation of PgDREB2A in vitro by P. glaucum total cell extract occurred at threonine residue(s). The phosphorylated PgDREB2A did not bind to the DREs. The present data indicate that stress induction of genes could occur via post-translational modification by phosphorylation of DREB2A.
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