Trial reportMolecular medicine (Cambridge, Mass.)
Limited dynamic range of immune response gene expression observed in healthy blood donors using RT-PCR.
Trial report in Molecular medicine (Cambridge, Mass.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 40 citations in OpenAlex.
- Dynamic expression of leukocyte innate immune genes in whole blood from horses with lipopolysaccharide-induced acute systemic inflammation.BMC veterinary research · 2015Trial
- Baseline Gene Expression Levels in Falkland-Malvinas Island Penguins: Towards a New Monitoring Paradigm.Life (Basel, Switzerland) · 2022Article
- Using transcriptomics to predict and visualize disease status in bighorn sheep (Conservation physiology · 2022Article
- Gene Expression Profiles in Two Razor Clam Populations: Discerning Drivers of Population Status.Life (Basel, Switzerland) · 2021Article
- Lipoprotein-associated phospholipase A2: The story continues.Medicinal research reviews · 2020Review
- Monitoring nearshore ecosystem health using Pacific razor clams (Siliqua patula) as an indicator species.PeerJ · 2020Article
- Physiological and gene transcription assays to assess responses of mussels to environmental changes.PeerJ · 2019Article
- Using Gene Transcription to Assess Ecological and Anthropological Stressors in Brown Bears.EcoHealth · 2018Article
- Coupling gene-based and classic veterinary diagnostics improves interpretation of health and immune function in the Agassiz's desert tortoise (Conservation physiology · 2017Article
- Integrating Gene Transcription-Based Biomarkers to Understand Desert Tortoise and Ecosystem Health.EcoHealth · 2015Article
- Improving power to detect changes in blood miRNA expression by accounting for sources of variability in experimental designs.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2014Article
- Blood-based gene expression signatures of infants and toddlers with autism.Journal of the American Academy of Child and Adolescent Psychiatry · 2012Article
- Combined anti-tumor necrosis factor-α therapy and DMARD therapy in rheumatoid arthritis patients reduces inflammatory gene expression in whole blood compared to DMARD therapy alone.Frontiers in immunology · 2012Article
- Correlation of pharmacodynamic activity, pharmacokinetics, and anti-product antibody responses to anti-IL-21R antibody therapeutics following IV administration to cynomolgus monkeys.Journal of translational medicine · 2010Article
- Increased cytokine production in interleukin-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling.The Journal of biological chemistry · 2009Article
- A longitudinal study of gene expression in healthy individuals.BMC medical genomics · 2009Article
- Alpha-1-antitrypsin is an endogenous inhibitor of proinflammatory cytokine production in whole blood.Journal of leukocyte biology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of quantitative gene expression analysis for the diagnosis, prognosis, and monitoring of disease requires the ability to distinguish pathophysiological changes from natural variations. To characterize these variations in apparently healthy subjects, quantitative real-time PCR was used to measure various immune response genes in whole blood collected from blood bank donors. In a single-time-point study of 131 donors, of 48 target genes, 43 were consistently expressed and 34 followed approximately log-normal distribution. Most transcripts showed a limited dynamic range of expression across subjects. Specifically, 36 genes had standard deviations (SDs) of 0.44 to 0.79 cycle threshold (C(T)) units, corresponding to less than a 3-fold variation in expression. Separately, a longitudinal study of 8 healthy individuals demonstrated a total dynamic range (> 2 standard error units) of 2- to 4-fold in most genes. In contrast, a study of whole blood gene expression in 6 volunteers injected with LPS showed 15 genes changing in expression 10- to 90-fold within 2 to 5 h and returning to within normal range within 21 hours. This work demonstrates that (1) the dynamic range of expression of many immune response genes is limited among healthy subjects; (2) expression levels for most genes analyzed are approximately log-normally distributed; and (3) individuals exposed to an infusion of bacterial endotoxin (lipopolysaccharide), show gene expression profiles that can be readily distinguished from those of a healthy population. These results suggest that normal reference ranges can be established for gene expression assays, providing critical standards for the diagnosis and management of disease.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.