ArticleInfection and immunity2007
Differences in Chlamydia trachomatis serovar E growth rate in polarized endometrial and endocervical epithelial cells grown in three-dimensional culture.
Article in Infection and immunity, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 34 citations in OpenAlex.
- Repurposing epetraborole to combatAntimicrobial agents and chemotherapy · 2026Article
- Epithelial polarity: a key player inmicroLife · 2026Review
- Dectin-1 stimulating β-glucans inhibit Chlamydia infections both in vitro and in vivo.Pathogens and disease · 2025Article
- HtrA, fatty acids, and membrane protein interplay inJournal of bacteriology · 2024Article
- Better In Vitro Tools for ExploringLife (Basel, Switzerland) · 2022Article
- Review
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- An endometrial organoid model of interactions betweenJournal of cell science · 2021Article
- Organoid technology in female reproductive biomedicine.Reproductive biology and endocrinology : RB&E · 2020Review
- Murine Endometrial Organoids to ModelFrontiers in cellular and infection microbiology · 2020Article
- The T-Cell Response to Type 2 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV).Viruses · 2019Article
- A renewed tool kit to exploreF1000Research · 2019Review
- Development of a NovelFrontiers in cellular and infection microbiology · 2019Article
- Optimal Stem Cell Transporting Conditions to Maintain Cell Viability and Characteristics.Tissue engineering and regenerative medicine · 2018Article
- Binding of Elementary Bodies by the Opportunistic Fungal PathogenFrontiers in microbiology · 2018Article
- Inhibition of Wnt Signaling Pathways ImpairsFrontiers in cellular and infection microbiology · 2017Article
- Effects of Yeast and Bacterial Commensals and Pathogens of the Female Genital Tract on the Transepithelial Electrical Resistance of HeLa Cells.The open microbiology journal · 2016Article
- Water-filtered infrared a irradiation in combination with visible light inhibits acute chlamydial infection.PloS one · 2014Article
- Chlamydia muridarum enters a viable but non-infectious state in amoxicillin-treated BALB/c mice.Microbes and infection · 2012Article
- Organotypic 3D cell culture models: using the rotating wall vessel to study host-pathogen interactions.Nature reviews. Microbiology · 2010Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
In vitro studies of obligate intracellular chlamydia biology and pathogenesis are highly dependent on the use of experimental models and growth conditions that mimic the mucosal architecture and environment these pathogens encounter during natural infections. In this study, the growth of Chlamydia trachomatis genital serovar E was monitored in mouse fibroblast McCoy cells and compared to more relevant host human epithelial endometrium-derived HEC-1B and cervix-derived HeLa cells, seeded and polarized on collagen-coated microcarrier beads, using a three-dimensional culture system. Microscopy analysis of these cell lines prior to infection revealed morphological differences reminiscent of their in vivo architecture. Upon infection, early chlamydial inclusion distribution was uniform in McCoy cells but patchy in both epithelial cell lines. Although no difference in chlamydial attachment to or entry into the two genital epithelial cell lines was noted, active bacterial genome replication and transcription, as well as initial transformation of elementary bodies to reticulate bodies, were detected earlier in HEC-1B than in HeLa cells, suggesting a faster growth, which led to higher progeny counts and titers in HEC-1B cells upon completion of the developmental cycle. Chlamydial development in the less relevant McCoy cells was very similar to that in HeLa cells, although higher progeny counts were obtained. In conclusion, this three-dimensional bead culture system represents an improved model for harvesting large quantities of infectious chlamydia progeny from their more natural polarized epithelial host cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.