ArticleNucleic acids research2006
The involvement of replication in single stranded oligonucleotide-mediated gene repair.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- SETDB1/ATF7IP regulate the precise genome engineering of HUSH-regulated genes.Epigenetics & chromatin · 2026Article
- Harnessing CRISPR-Cas9 for Lactobacillus improvement in silage production: current knowledge and future perspectives.Journal of animal science and biotechnology · 2025Review
- Emerging tools in plant genome editing.Frontiers in genome editing · 2025Review
- Reducing competition between msd and genomic DNA improves retron editing efficiency.EMBO reports · 2024Article
- Extensive trimming of short single-stranded DNA oligonucleotides during replication-coupled gene editing in mammalian cells.PLoS genetics · 2020Article
- CRISPR-DirectedThe CRISPR journal · 2018Article
- Relaxed chromatin induced by histone deacetylase inhibitors improves the oligonucleotide-directed gene editing in plant cells.Journal of plant research · 2018Article
- Mechanisms of precise genome editing using oligonucleotide donors.Genome research · 2017Article
- Article
- λ Recombination and Recombineering.EcoSal Plus · 2016Review
- LNA modification of single-stranded DNA oligonucleotides allows subtle gene modification in mismatch-repair-proficient cells.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Replicative DNA polymerase δ but not ε proofreads errors in Cis and in Trans.PLoS genetics · 2015Article
- Regulation of Gene Editing Activity Directed by Single-Stranded Oligonucleotides and CRISPR/Cas9 Systems.PloS one · 2015Article
- Lambda red mediated gap repair utilizes a novel replicative intermediate in Escherichia coli.PloS one · 2015Article
- Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations.Science (New York, N.Y.) · 2014Article
- Combinatorial gene editing in mammalian cells using ssODNs and TALENs.Scientific reports · 2014Article
- Article
- Recombination promoted by DNA viruses: phage λ to herpes simplex virus.Annual review of microbiology · 2014Review
- CRISPR-Cas9-assisted recombineering in Lactobacillus reuteri.Nucleic acids research · 2014Article
- Bacterial DNA polymerases participate in oligonucleotide recombination.Molecular microbiology · 2013Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Targeted gene repair mediated by single-stranded oligonucleotides (SSOs) has great potential for use in functional genomic studies and gene therapy. Genetic changes have been created using this approach in a number of prokaryotic and eukaryotic systems, including mouse embryonic stem cells. However, the underlying mechanisms remain to be fully established. In one of the current models, the 'annealing-integration' model, the SSO anneals to its target locus at the replication fork, serving as a primer for subsequent DNA synthesis mediated by the host replication machinery. Using a lambda-Red recombination-based system in the bacterium Escherichia coli, we systematically examined several fundamental premises that form the mechanistic basis of this model. Our results provide direct evidence strongly suggesting that SSO-mediated gene repair is mechanistically linked to the process of DNA replication, and most likely involves a replication intermediate. These findings will help guide future experiments involving SSO-mediated gene repair in mammalian and prokaryotic cells, and suggest several mechanisms by which the efficiencies may be reliably and substantially increased.
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Registered trials
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