ArticleInternational journal for parasitology2007
Clathrin-dependent pathways and the cytoskeleton network are involved in ceramide endocytosis by a parasitic protozoan, Giardia lamblia.
Article in International journal for parasitology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 48 citations in OpenAlex.
- Genotype-Specific Small EVs Released by Giardia lamblia Act as Mediators of Phenotypic Adaptation Under Metronidazole-Induced Stress.Journal of extracellular vesicles · 2025Article
- Giardiavirus infection alleviates growth restriction and intestinal damage caused by the intestinal parasite Giardia duodenalis.Parasites & vectors · 2025Article
- Endocytosis in anaerobic parasitic protists.Memorias do Instituto Oswaldo Cruz · 2024Review
- Ultrastructural Alterations of the Human PathogenPathogens (Basel, Switzerland) · 2023Review
- Lipidome of extracellular vesicles from Giardia lamblia.PloS one · 2023Article
- Unusual Cell Structures and Organelles inMicroorganisms · 2022Review
- Article
- Exosome Biogenesis in the Protozoa ParasiteCells · 2019Article
- Lactoferrin and lactoferricin endocytosis halt Giardia cell growth and prevent infective cyst production.Scientific reports · 2018Article
- Glucosylceramide transferase in Giardia preferentially catalyzes the synthesis of galactosylceramide during encystation.Molecular and biochemical parasitology · 2017Article
- Use of fluorescent nanoparticles to investigate nutrient acquisition by developing Eimeria maxima macrogametocytes.Scientific reports · 2016Article
- Sphingolipids, Lipid Rafts, and Giardial Encystation: The Show Must Go On.Current tropical medicine reports · 2015Article
- Binding and Endocytosis of Bovine Hololactoferrin by the Parasite Entamoeba histolytica.BioMed research international · 2015Article
- Glucosylceramide transferase activity is critical for encystation and viable cyst production by an intestinal protozoan, Giardia lamblia.The Journal of biological chemistry · 2013Article
- Lysosomal protein trafficking in Giardia lamblia: common and distinct features.Frontiers in bioscience (Elite edition) · 2012Review
- Lipid metabolism in Giardia: a post-genomic perspective.Parasitology · 2011Review
- Glucosylceramide synthesis inhibition affects cell cycle progression, membrane trafficking, and stage differentiation in Giardia lamblia.Journal of lipid research · 2010Article
- Rab11 and actin cytoskeleton participate in Giardia lamblia encystation, guiding the specific vesicles to the cyst wall.PLoS neglected tropical diseases · 2010Article
- Adaptor protein 2 regulates receptor-mediated endocytosis and cyst formation in Giardia lamblia.The Biochemical journal · 2010Article
- A contiguous compartment functions as endoplasmic reticulum and endosome/lysosome in Giardia lamblia.Eukaryotic cell · 2009Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Although identified as an early-diverged protozoan, Giardia lamblia shares many similarities with higher eukaryotic cells, including an internal membrane system and cytoskeleton, as well as secretory pathways. However, unlike many other eukaryotes, Giardia does not synthesize lipids de novo, but rather depends on exogenous sources for both energy production and organelle or membrane biogenesis. It is not known how lipid molecules are taken up by this parasite and if endocytic pathways are involved in this process. In this investigation, we tested the hypothesis that highly regulated and selective lipid transport machinery is present in Giardia and necessary for the efficient internalization and intracellular targeting of ceramide molecules, the major sphingolipid precursor. Using metabolic and pathway inhibitors, we demonstrate that ceramide is internalized through endocytic pathways and is primarily targeted into perinuclear/endoplasmic reticulum membranes. Further investigations suggested that Giardia uses both clathrin-dependent pathways and the actin cytoskeleton for ceramide uptake, as well as microtubule filaments for intracellular localization and targeting. We speculate that this parasitic protozoan has evolved cytoskeletal and clathrin-dependent endocytic mechanisms for importing ceramide molecules from the cell exterior for the synthesis of membranes and vesicles during growth and differentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.