ArticleCellular microbiology2007
Novel replication complex architecture in rubella replicon-transfected cells.
Article in Cellular microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 56 citations in OpenAlex.
- SiScience · 2023Article
- A conserved viral amphipathic helix governs the replication site-specific membrane association.PLoS pathogens · 2022Article
- Exogenous Rubella Virus Capsid Proteins Enhance Virus Genome Replication.Pathogens (Basel, Switzerland) · 2022Article
- Ultrastructural Features of Membranous Replication Organelles Induced by Positive-Stranded RNA Viruses.Cells · 2021Review
- Molecular and Structural Insights into the Life Cycle of Rubella Virus.Journal of virology · 2021Review
- Article
- A modified lysosomal organelle mediates nonlytic egress of reovirus.The Journal of cell biology · 2020Article
- Molecular aspects of the teratogenesis of rubella virus.Biological research · 2019Review
- The viral replication organelles within cells studied by electron microscopy.Advances in virus research · 2019Article
- Article
- ESCRT machinery components are required for Orthobunyavirus particle production in Golgi compartments.PLoS pathogens · 2018Article
- Antiviral activity of hemolymph of Podalia against rubella virus.Cytotechnology · 2017Article
- (+) RNA virus replication compartments: a safe home for (most) viral replication.Current opinion in microbiology · 2016Review
- Review
- Co-opted oxysterol-binding ORP and VAP proteins channel sterols to RNA virus replication sites via membrane contact sites.PLoS pathogens · 2014Article
- Review
- Noncanonical role for the host Vps4 AAA+ ATPase ESCRT protein in the formation of Tomato bushy stunt virus replicase.PLoS pathogens · 2014Article
- Reovirus forms neo-organelles for progeny particle assembly within reorganized cell membranes.mBio · 2014Article
- Architecture and biogenesis of plus-strand RNA virus replication factories.World journal of virology · 2013Review
- How to get out: ssRNA enveloped viruses and membrane fission.Current opinion in virology · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Rubella virus (RUB) assembles its replication complexes (RCs) in modified organelles of endo-lysosomal origin, known as cytopathic vacuoles (CPVs). These peculiar structures are key elements of RUB factories, where rough endoplasmic reticulum, mitochondria, and Golgi are recruited. Bicistronic RUB replicons expressing an antibiotic resistance gene either in the presence or the absence of the RUB capsid (C) gene were used to study the structure of RCs in transfected cells. Confocal microscopy showed that the RUB replicase components P90 and P150 localized to CPVs, as did double-stranded RNA (dsRNA), a marker for RNA synthesis. Electron microscopy (EM) showed that replicons generated CPVs containing small vesicles and large vacuoles, similar to CPVs from RUB-infected cells and that the replicase proteins were sufficient for organelle recruitment. Some of these CPVs contained straight membranes. When cross-sectioned, these rigid membranes appeared to be sheets of closely packed proteins. Immuno-EM revealed that these sheets, apparently in contact with the cytosol, contained both P150 and P90, as well as dsRNA, and thus could be two-dimensional arrays of functional viral replicases. Labelling of dsRNA after streptolysin-O permeabilization showed that replication of viral genome takes place on the cytoplasmic side of CPVs. When present, C accumulated around CPVs. Mitochondrial protein P32 was detected within modified CPVs, the first demonstration of involvement of this protein, which interacts with C, with RCs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.