ArticleApplied and environmental microbiology2007
Multiple-level regulation of genes for protocatechuate degradation in Acinetobacter baylyi includes cross-regulation.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 25 citations in OpenAlex.
- Predicted Bacterial Metabolic Landscapes of the Sumaco Volcano: A Picrust2 Analysis of 16S rRNA Data from Amazonian Ecuador.Microorganisms · 2026Article
- Comparative Analysis of the IclR-Family of Bacterial Transcription Factors and Their DNA-Binding Motifs: Structure, Positioning, Co-Evolution, Regulon Content.Frontiers in microbiology · 2021Article
- Microbial Metabolic Potential of Phenol Degradation in Wastewater Treatment Plant of Crude Oil Refinery: Analysis of Metagenomes and Characterization of Isolates.Microorganisms · 2020Article
- De novo structure determination of 3-((3-aminopropyl)amino)-4-hydroxybenzoic acid, a novel and abundant metabolite in Acinetobacter baylyi ADP1.Metabolomics : Official journal of the Metabolomic Society · 2019Article
- Cloning, expression, crystallization and crystallographic analysis of CouR from Rhodopseudomonas palustris.Acta crystallographica. Section F, Structural biology communications · 2015Article
- Novel metabolic features inMetabolomics : Official journal of the Metabolomic Society · 2014Article
- Simultaneous catabolism of plant-derived aromatic compounds results in enhanced growth for members of the Roseobacter lineage.Applied and environmental microbiology · 2013Article
- Study of PcaV from Streptomyces coelicolor yields new insights into ligand-responsive MarR family transcription factors.Nucleic acids research · 2013Article
- PcaO positively regulates pcaHG of the beta-ketoadipate pathway in Corynebacterium glutamicum.Journal of bacteriology · 2010Article
- Regulation of expression of genes involved in quinate and shikimate utilization in Corynebacterium glutamicum.Applied and environmental microbiology · 2009Article
- Role of Acinetobacter baylyi Crc in catabolite repression of enzymes for aromatic compound catabolism.Journal of bacteriology · 2009Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bacterium Acinetobacter baylyi uses the branched beta-ketoadipate pathway to metabolize aromatic compounds. Here, the multiple-level regulation of expression of the pca-qui operon encoding the enzymes for protocatechuate and quinate degradation was studied. It is shown that both activities of the IclR-type regulator protein PcaU at the structural gene promoter pcaIp, namely protocatechuate-dependent activation of pca-qui operon expression as well as repression in the absence of protocatechuate, can be observed in a different cellular background (Escherichia coli) and therefore are intrinsic to PcaU. The regulation of PcaU expression is demonstrated to be carbon source dependent according to the same pattern as the pca-qui operon. The increase of the pcaU gene copy number leads to a decrease of the basal expression at pcaIp, indicating that the occupancy of the PcaU binding site is well balanced and depends on the concentration of PcaU in the cell. Luciferase is used as a reporter to demonstrate strong repression of pcaIp when benzoate, a substrate of the catechol branch of the pathway, is present in addition to substrates of the protocatechuate branch (cross-regulation). The same repression pattern was observed for promoter pcaUp. Thus, three promoters involved in gene expression of enzymes of the protocatechuate branch (pobAp upstream of pobA, pcaIp, and pcaUp) are strongly repressed in the presence of benzoate. The negative effect of protocatechuate on pobA expression is not based on a direct sensing of the metabolite by PobR, the specific regulator of pobA expression.
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