ArticleApplied and environmental microbiology2007
Procedure for rapid concentration and detection of enteric viruses from berries and vegetables.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 181 citations in OpenAlex.
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- Development of an Extraction Method to Detect Hepatitis A Virus, Hepatitis E Virus, and Noroviruses in Fish Products.Microorganisms · 2023Article
- New insights in pectinase production development and industrial applications.Applied microbiology and biotechnology · 2021Review
- Enhancement of the Molecular and Serological Assessment of Hepatitis E Virus in Milk Samples.Microorganisms · 2020Article
- Discovery of Components Acting as the Obstacles in the Detection of Enteric Viruses from Berries.Food and environmental virology · 2020Article
- Relevance of SARS-CoV-2 in food safety and food hygiene: potential preventive measures, suggestions and nanotechnological approaches.Virusdisease · 2020Review
- The use of bacteriophage MS2 for the development and application of a virucide decontamination test method for porous and heavily soiled surfaces.Journal of applied microbiology · 2019Article
- A rapid method for separating and concentration of food-borne pathogens using elution from ready-to-eat vegetables.Iranian journal of microbiology · 2018Article
- Rapid Detection of Human Norovirus in Frozen Raspberries.Food and environmental virology · 2018Article
- Preliminary Source Tracking of Male-Specific (FFood and environmental virology · 2017Article
- Development of a PCR-free DNA-based assay for the specific detection of Vibrio species in environmental samples by targeting the 16S rRNA.Environmental science and pollution research international · 2017Article
- Evaluation of Molecular Methods for the Detection and Quantification of Pathogen-Derived Nucleic Acids in Sediment.Frontiers in microbiology · 2017Article
- Identification of Enteric Viruses in Foods from Mexico City.Food and environmental virology · 2016Article
- Application of F⁺RNA Coliphages as Source Tracking Enteric Viruses on Parsley and Leek Using RT-PCR.Food and environmental virology · 2015Article
- Development of a Practical Method to Detect Noroviruses Contamination in Composite Meals.Food and environmental virology · 2015Article
- Possible Internalization of an Enterovirus in Hydroponically Grown Lettuce.International journal of environmental research and public health · 2015Article
- Prevalence of human noroviruses in frozen marketed shellfish, red fruits and fresh vegetables.Food and environmental virology · 2014Article
- A rapid single-tube protocol for HAV detection by nested real-time PCR.Food and environmental virology · 2014Article
- HAV in fresh vegetables: a hidden health risk in district Mardan, Pakistan.SpringerPlus · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several hepatitis A virus (HAV) and norovirus (NV) outbreaks due to consumption of berries and vegetables have been reported during recent years. To facilitate the detection of enteric viruses that may be present on different fresh and frozen products, we developed a rapid and sensitive detection method for HAV, NV, and rotavirus (RV). Initial experiments focused on optimizing the composition of the elution buffer, improving the viral concentration method, and evaluating the performance of various extraction kits. Viruses were extracted from the food surface by a direct elution method in a glycine-Tris (pH 9.5) buffer containing 1% beef extract and concentrated by ultrafiltration. Occasionally, PCR inhibitors were present in the processed berry samples, which gave relatively poor detection limits. However, this problem was overcome by adding a pectinase treatment in the protocol, which markedly improved the sensitivity of the method. After optimization, this concentration method was applied in combination with real-time reverse transcription-PCR (RT-PCR) using specific primers in various types of berries and vegetables. The average detection limits were 1 50% tissue culture infective dose (TCID(50)), 54 RT-PCR units, and 0.02 TCID(50) per 15 g of food for HAV, NV, and RV, respectively. Based on our results, it is concluded that this procedure is suitable to detect and quantify enteric viruses within 6 h and can be applied for surveillance of enteric viruses in fresh and frozen products.
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