ArticleApplied and environmental microbiology2007
A single-nucleotide-polymorphism-based multilocus genotyping assay for subtyping lineage I isolates of Listeria monocytogenes.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Bioprotective Strategies to ControlInternational journal of molecular sciences · 2025Review
- Serotype-identifying ions inHeliyon · 2022Article
- Single-nucleotide polymorphism-based epidemiological analysis of KoreanJournal of veterinary science · 2021Article
- A review of Listeria monocytogenes from meat and meat products: Epidemiology, virulence factors, antimicrobial resistance and diagnosis.The Onderstepoort journal of veterinary research · 2020Review
- Population Structure of Non-ST6Microorganisms · 2020Article
- Genetic diversity of Listeria monocytogenes strains in ruminant abortion and rhombencephalitis cases in comparison with the natural environment.BMC microbiology · 2019Article
- Genomic Diversity of Common Sequence Types ofGenes · 2019Article
- Article
- The Arsenic Resistance-Associated Listeria Genomic Island LGI2 Exhibits Sequence and Integration Site Diversity and a Propensity for Three Listeria monocytogenes Clones with Enhanced Virulence.Applied and environmental microbiology · 2017Article
- Subtyping of Salmonella enterica Subspecies I Using Single-Nucleotide Polymorphisms in Adenylate Cyclase.Foodborne pathogens and disease · 2016Article
- Article
- DNase-Sensitive and -Resistant Modes of Biofilm Formation by Listeria monocytogenes.Frontiers in microbiology · 2015Article
- Population structure of Listeria monocytogenes serotype 4b isolates from sporadic human listeriosis cases in the United States from 2003 to 2008.Applied and environmental microbiology · 2014Article
- "Epidemic clones" of Listeria monocytogenes are widespread and ancient clonal groups.Journal of clinical microbiology · 2013Article
- Molecular approaches to the identification of pathogenic and nonpathogenic listeriae.Microbiology insights · 2013Review
- Heavy metal and disinfectant resistance of Listeria monocytogenes from foods and food processing plants.Applied and environmental microbiology · 2012Article
- Comparison of the Potency of the Lipid II Targeting Antimicrobials Nisin, Lacticin 3147 and Vancomycin Against Gram-Positive Bacteria.Probiotics and antimicrobial proteins · 2012Article
- Two novel type II restriction-modification systems occupying genomically equivalent locations on the chromosomes of Listeria monocytogenes strains.Applied and environmental microbiology · 2012Article
- A novel restriction-modification system is responsible for temperature-dependent phage resistance in Listeria monocytogenes ECII.Applied and environmental microbiology · 2012Article
- Atypical Listeria monocytogenes serotype 4b strains harboring a lineage II-specific gene cassette.Applied and environmental microbiology · 2012Article
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6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Listeria monocytogenes is a facultative intracellular pathogen responsible for food-borne disease with high mortality rates in humans and is the leading microbiological cause of food recalls. Lineage I isolates of L. monocytogenes are a particular public health concern because they are responsible for most sporadic cases of listeriosis and the vast majority of epidemic outbreaks. Rapid, reproducible, and sensitive methods for differentiating pathogens below the species level are required for effective pathogen control programs, and the CDC PulseNet Task Force has called for the development and validation of DNA sequence-based methods for subtyping food-borne pathogens. Therefore, we developed a multilocus genotyping (MLGT) assay for L. monocytogenes lineage I isolates based on nucleotide variation identified by sequencing 23,251 bp of DNA from 22 genes distributed across seven genomic regions in 65 L. monocytogenes isolates. This single-well assay of 60 allele-specific probes captured 100% of the haplotype information contained in approximately 1.5 Mb of comparative DNA sequence and was used to reproducibly type a total of 241 lineage I isolates. The MLGT assay provided high discriminatory power (Simpson's index value, 0.91), uniquely identified isolates from the eight listeriosis outbreaks examined, and differentiated serotypes 1/2b and 4b as well as epidemic clone I (ECI), ECIa, and ECII. In addition, the assay included probes for a previously characterized truncation mutation in inlA, providing for the identification of a specific virulence-attenuated subtype. These results demonstrate that MLGT represents a significant new tool for use in pathogen surveillance, outbreak detection, risk assessment, population analyses, and epidemiological investigations. DNA sequences were deposited in the GenBank database under accession numbers DQ 812146 to DQ 812517, DQ 843664 to DQ 844598, and AY 512391 to AY 512502.
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