ArticleApplied and environmental microbiology2007
Molecular analysis of the subgingival microbiota in health and disease.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers, 1 of them a synthesis that pooled it.
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Who cites it
63 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Newly identified pathogens associated with periodontitis: a systematic review.Journal of dental research · 2014Pooled it
- EBV promotes alveolar trabecula resorption via extracellular vesicle remodeling by group IIA secreted phospholipase AJournal of lipid research · 2026Article
- Comparative pilot study of three commercial kits for bacterial DNA extraction from human subgingival biofilm samples collected with a single paper point.Journal of oral microbiology · 2025Article
- Epstein-Barr Virus Promotes Inflammatory Cytokine Production in Human Gingival Fibroblasts.International dental journal · 2024Article
- The gut-brain-axis: A positive relationship between gut microbial dysbiosis and glioblastoma brain tumour.Heliyon · 2024Article
- Comparison of the oral microbial composition between healthy individuals and periodontitis patients in different oral sampling sites using 16S metagenome profiling.Journal of periodontal & implant science · 2022Article
- Epstein-Barr Virus Promotes the Production of Inflammatory Cytokines in Gingival Fibroblasts and RANKL-Induced Osteoclast Differentiation in RAW264.7 Cells.International journal of molecular sciences · 2022Article
- Gut-Thyroid axis: How gut microbial dysbiosis associated with euthyroid thyroid cancer.Journal of Cancer · 2022Article
- Pre and Probiotics Involved in the Modulation of Oral Bacterial Species: New Therapeutic Leads in Mental Disorders?Microorganisms · 2021Review
- Analysing the Relationship between Nutrition and the Microbial Composition of the Oral Biofilm-Insights from the Analysis of Individual Variability.Antibiotics (Basel, Switzerland) · 2020Article
- How Does Epstein-Barr Virus Contribute to Chronic Periodontitis?International journal of molecular sciences · 2020Review
- The microbiome: Composition and locations.Progress in molecular biology and translational science · 2020Review
- Shockwave Therapy Efficiently Cures Multispecies Chronic Periodontitis in a Humanized Rat Model.Frontiers in bioengineering and biotechnology · 2019Article
- Fecal microbiota composition changes after a BW loss diet in Beagle dogs.Journal of animal science · 2018Article
- Dynamic changes of bacterial communities and nitrite character during northeastern Chinese sauerkraut fermentation.Food science and biotechnology · 2018Article
- Oral Biofilm Sampling for Microbiome Analysis in Healthy Children.Journal of visualized experiments : JoVE · 2017Article
- In Situ Anabolic Activity of Periodontal Pathogens Porphyromonas gingivalis and Filifactor alocis in Chronic Periodontitis.Scientific reports · 2016Article
- Metabolomic Studies of Oral Biofilm, Oral Cancer, and Beyond.International journal of molecular sciences · 2016Review
- Levels of Candidate Periodontal Pathogens in Subgingival Biofilm.Journal of dental research · 2016Article
- Periodontal pathogens and tetracycline resistance genes in subgingival biofilm of periodontally healthy and diseased Dominican adults.Clinical oral investigations · 2016Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This investigation provides molecular analyses of the periodontal microbiota in health and disease. Subgingival samples from 47 volunteers with healthy gingivae or clinically diagnosed chronic periodontitis were characterized by PCR-denaturing gradient gel electrophoresis (DGGE) with primers specific for the V2-V3 region of the eubacterial 16S rRNA gene. A hierarchical dendrogram was constructed from band patterns. All unique PCR amplicons (DGGE bands) were sequenced for identity. Samples were also analyzed for the presence of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and Tannerella forsythensis by multiplex PCR. Associations of patient age, gender, and smoking status together with the presence of each unique band and putative periodontal pathogens with disease were assessed by logistic regression. Periodontal pockets were colonized by complex eubacterial communities (10 to 40 distinct DGGE bands) with substantial individual variation in the community profile. Species diversity in health and disease was determined by the Shannon-Weaver index of diversity and compared by the Mann-Whitney U test. Sequence analyses of DGGE amplicons indicated the occurrence of many nontypical oral species and eubacteria previously associated with this environment. With the exception of T. forsythensis, the putative pathogens were not detected by DGGE. Multiplex PCR, however, detected T. forsythensis, A. actinomycetemcomitans, and P. gingivalis in 9% 16%, and 29% of the patients with disease, respectively. The presence of A. actinomycetemcomitans was significantly associated with disease (P < 0.01). Statistical analyses indicated that the presence of Treponema socranskii and Pseudomonas sp. was a significant predictor of disease (P < 0.05) and that there was no significant difference (P > 0.05) in terms of eubacterial species diversity between health and disease.
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Registered trials
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