ArticleProceedings of the National Academy of Sciences of the United States of America2006
Telomere-mediated chromosomal truncation in maize.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- SEM mapping of sequence-specific protein-DNA interactions on long DNA molecules.Nucleic acids research · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Metal- and polymer-assisted SEM imaging of protein-bound DNA molecules: a protocol.Biology methods & protocols · 2026Review
- Past innovations and future possibilities in plant chromosome engineering.Plant biotechnology journal · 2025Review
- Recent advances in site-specific transgene insertion into the maize genome using recombinases and genome editing endonucleases.Frontiers in plant science · 2025Review
- Rice transformation treatments leave specific epigenome changes beyond tissue culture.Plant physiology · 2023Article
- Towards Plant Synthetic Genomics.Biodesign research · 2023Article
- Whole Genome Sequencing Analysis of Effects of CRISPR/Cas9 inJournal of fungi (Basel, Switzerland) · 2022Article
- Telomeres and Their Neighbors.Genes · 2022Review
- Distinct mechanisms for genomic attachment of the 5' and 3' ends of Agrobacterium T-DNA in plants.Nature plants · 2022Article
- Biocircuits in plants and eukaryotic algae.Frontiers in plant science · 2022Review
- Artificial chromosome technology and its potential application in plants.Frontiers in plant science · 2022Review
- Precise Characterization and Tracking of Stably Inherited Artificial Minichromosomes Made by Telomere-Mediated Chromosome Truncation inFrontiers in plant science · 2021Article
- Transposon-mediated telomere destabilization: a driver of genome evolution in the blast fungus.Nucleic acids research · 2020Article
- Genetic and epigenetic effects on centromere establishment.Chromosoma · 2020Review
- James A. Birchler.The Plant cell · 2019Article
- Unbiased K-mer Analysis Reveals Changes in Copy Number of Highly Repetitive Sequences During Maize Domestication and Improvement.Scientific reports · 2017Article
- Plant artificial chromosome technology and its potential application in genetic engineering.Plant biotechnology journal · 2016Review
- Stable mitotic inheritance of rice minichromosomes in cell suspension cultures.Plant cell reports · 2015Article
- Engineering of plant chromosomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
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4 authors.
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Abstract
Direct repeats of Arabidopsis telomeric sequence were constructed to test telomere-mediated chromosomal truncation in maize. Two constructs with 2.6 kb of telomeric sequence were used to transform maize immature embryos by Agrobacterium-mediated transformation. One hundred seventy-six transgenic lines were recovered in which 231 transgene loci were revealed by a FISH analysis. To analyze chromosomal truncations that result in transgenes located near chromosomal termini, Southern hybridization analyses were performed. A pattern of smear in truncated lines was seen as compared with discrete bands for internal integrations, because telomeres in different cells are elongated differently by telomerase. When multiple restriction enzymes were used to map the transgene positions, the size of the smears shifted in accordance with the locations of restriction sites on the construct. This result demonstrated that the transgene was present at the end of the chromosome immediately before the integrated telomere sequence. Direct evidence for chromosomal truncation came from the results of FISH karyotyping, which revealed broken chromosomes with transgene signals at the ends. These results demonstrate that telomere-mediated chromosomal truncation operates in plant species. This technology will be useful for chromosomal engineering in maize as well as other plant species.
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