ArticleProceedings of the National Academy of Sciences of the United States of America2006
SK channels mediate NADPH oxidase-independent reactive oxygen species production and apoptosis in granulocytes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- SK3 channel and mitochondrial ROS mediate NADPH oxidase-independent NETosis induced by calcium influx.Proceedings of the National Academy of Sciences of the United States of America · 2015Trial
- Neutrophil extracellular traps in liver diseases: from molecular mechanisms to therapeutic interventions.Acta pharmacologica Sinica · 2026Review
- Neutrophil Extracellular DNA Traps in Response to Infection or Inflammation, and the Roles of Platelet Interactions.International journal of molecular sciences · 2024Review
- Mechanisms of Neutrophil Extracellular Trap Formation and Regulation in Cancers.International journal of molecular sciences · 2023Review
- Neutrophil extracellular traps mediate deep vein thrombosis: from mechanism to therapy.Frontiers in immunology · 2023Review
- The role of neutrophil extracellular traps in cancer progression, metastasis and therapy.Experimental hematology & oncology · 2022Review
- Neutrophils' Extracellular Trap Mechanisms: From Physiology to Pathology.International journal of molecular sciences · 2022Review
- Activating Parkin-dependent mitophagy alleviates oxidative stress, apoptosis, and promotes random-pattern skin flaps survival.Communications biology · 2022Article
- Synthesis, Biological Evaluation, and Molecular Modeling of Aza-Crown Ethers.Molecules (Basel, Switzerland) · 2021Article
- Neutrophils: Many Ways to Die.Frontiers in immunology · 2021Review
- Neutrophil Extracellular Trap Formation: Physiology, Pathology, and Pharmacology.Biomolecules · 2019Review
- Physiological Roles and Therapeutic Potential of CaFrontiers in molecular neuroscience · 2018Review
- Identity and function of a cardiac mitochondrial small conductance CaBiochimica et biophysica acta. Bioenergetics · 2017Article
- The complex cascade of cellular events governing inflammasome activation and IL-1β processing in response to inhaled particles.Particle and fibre toxicology · 2016Review
- Activation of the NLRP3/caspase-1 inflammasome in human dental pulp tissue and human dental pulp fibroblasts.Cell and tissue research · 2015Article
- Firing pattern modulation through SK channel current increase underlies neuronal survival in an organotypic slice model of Parkinson's disease.Molecular neurobiology · 2015Article
- KCa3.1-Dependent Hyperpolarization Enhances Intracellular Ca2+ Signaling Induced by fMLF in Differentiated U937 Cells.PloS one · 2015Article
- IL-1β drives inflammatory responses to propionibacterium acnes in vitro and in vivo.The Journal of investigative dermatology · 2014Article
- NLRP3 inflammasome activation induced by engineered nanomaterials.Small (Weinheim an der Bergstrasse, Germany) · 2013Review
- SKCa Channels Blockage Increases the Expression of Adenosine A2A Receptor in Jurkat Human T Cells.BioResearch open access · 2013Article
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Authors and funding
4 authors.
Funding
Abstract
Neutrophils are immune cells that bind to, engulf, and destroy bacterial and fungal pathogens in infected tissue, and their clearance by apoptosis is essential for the resolution of inflammation. Killing involves both oxidative and nonoxidative processes, the oxidative pathway requiring electrogenic production of superoxide by the membrane-bound NADPH oxidase complex. A variety of stimuli, from bacterial chemotactic peptides to complement- or IgG-opsonized microbes, can induce the production of reactive oxygen species (ROS) by neutrophils, presumably by means of NADPH oxidase. We report here that 1-ethyl-2-benzimidazolinone (1-EBIO), an activator of Ca2+-activated potassium channels of small conductance (SK) and intermediate conductance (IK), causes production of superoxide and hydrogen peroxide by neutrophils and granulocyte-differentiated PLB-985 cells. This response can be partially inhibited by the SK blocker apamin, which inhibits a Ca2+-activated K+ current in these cells. Analysis of RNA transcripts indicates that channels encoded by the SK3 gene carry this current. The effects of 1-EBIO and apamin are independent of the NADPH oxidase pathway, as demonstrated by using a PLB-985 cell line lacking the gp91phox subunit. Rather, 1-EBIO and apamin modulate mitochondrial ROS production. Consistent with the enhanced ROS production and K+ efflux mediated by 1-EBIO, we found that this SK opener increased apoptosis of PLB-985 cells. Together, these findings suggest a previously uncharacterized mechanism for the regulation of neutrophil ROS production and programmed cell death.
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