ArticleJournal of bacteriology2007
M.SpyI, a DNA methyltransferase encoded on a mefA chimeric element, modifies the genome of Streptococcus pyogenes.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- Eco-evolutionary feedbacks drive the co-occurrence of restriction-modification systems and antimicrobial resistance genes in bacteria.PLoS biology · 2026Article
- TheJournal of bacteriology · 2023Article
- Extension of bacterial rDNA sequencing for simultaneous methylation detection and its application in microflora analysis.Scientific reports · 2023Article
- Characterization of the Type I Restriction Modification System Broadly Conserved among Group A Streptococci.mSphere · 2021Article
- DNA methylation from a Type I restriction modification system influences gene expression and virulence in Streptococcus pyogenes.PLoS pathogens · 2019Article
- Phylogenetic relationship of prophages is affected by CRISPR selection in Group A Streptococcus.BMC microbiology · 2019Article
- Targeted Curing of All Lysogenic Bacteriophage from Streptococcus pyogenes Using a Novel Counter-selection Technique.PloS one · 2016Article
- Molecular epidemiology and genomics of group A Streptococcus.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2015Review
- Nucleotide sequence of conjugative prophage Φ1207.3 (formerly Tn1207.3) carrying the mef(A)/msr(D) genes for efflux resistance to macrolides in Streptococcus pyogenes.Frontiers in microbiology · 2014Article
- Phim46.1, the main Streptococcus pyogenes element carrying mef(A) and tet(O) genes.Antimicrobial agents and chemotherapy · 2010Article
- Population biology of the human restricted pathogen, Streptococcus pyogenes.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2009Review
- Association of the shuffling of Streptococcus pyogenes clones and the fluctuation of scarlet fever cases between 2000 and 2006 in central Taiwan.BMC microbiology · 2009Article
- Distribution of phage-associated virulence genes in pharyngeal group a streptococcal strains isolated in Italy.Journal of clinical microbiology · 2009Article
- Truncated human cytidylate-phosphate-deoxyguanylate-binding protein for improved nucleic acid amplification technique-based detection of bacterial species in human samples.Journal of clinical microbiology · 2009Article
- Genetic elements responsible for erythromycin resistance in streptococci.Antimicrobial agents and chemotherapy · 2009Review
- SmaI typeability and tetracycline susceptibility and resistance in Streptococcus pyogenes isolates with efflux-mediated erythromycin resistance.Antimicrobial agents and chemotherapy · 2007Article
- Novel algorithms reveal streptococcal transcriptomes and clues about undefined genes.PLoS computational biology · 2007Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
While screening the clonality of Streptococcus pyogenes isolates from an outbreak of erythromycin-resistant pharyngitis in Pittsburgh, PA, we found a correlation between the presence of the chimeric element Phi10394.4 (carrying the macrolide efflux gene, mefA) and genomic DNA being resistant to cleavage by SmaI restriction endonuclease. A search of the open reading frames in Phi10394.4 identified a putative type II restriction-modification (R-M) cassette containing a cytosine methyltransferase gene (spyIM). Heterologous expression of the cloned spyIM gene, as well as allelic-replacement experiments, showed that the action of this methyltransferase (M.SpyI) was responsible for the inhibition of SmaI digestion of genomic DNA in the Phi10394.4-containing isolates. Analysis of the methylation patterns of streptococcal genomic DNA from spyIM-positive strains, a spyIM deletion mutant, and a spyIM-negative strain determined that M.SpyI specifically recognized and methylated the DNA sequence to generate 5'-C(m)CNGG. To our knowledge, this is the first methyltransferase gene from S. pyogenes to be cloned and to have its activity characterized. These results reveal why pulsed field gel electrophoresis analysis of SmaI-digested genomic DNA cannot be used to analyze the clonality of some streptococci containing Phi10394.4 and may explain the inability of previous epidemiological studies to use SmaI to analyze DNAs from macrolide-resistant streptococci. The presence of the SpyI R-M cassette in Phi10394.4 could impart a selective advantage to host strain survival and may provide another explanation for the observed increase in macrolide-resistant streptococci.
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