ArticleJournal of bacteriology2007
Transcriptional regulation of the yghJ-pppA-yghG-gspCDEFGHIJKLM cluster, encoding the type II secretion pathway in enterotoxigenic Escherichia coli.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 47 citations in OpenAlex.
- Molecular Characterization of Multidrug-ResistantVeterinary sciences · 2024Article
- Membrane translocation process revealed by in situ structures of type II secretion system secretins.Nature communications · 2023Article
- Phylogenetic relationship and virulence composition ofFrontiers in microbiology · 2023Article
- Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation.NPJ biofilms and microbiomes · 2022Article
- Human Mucosal IgA Immune Responses against EnterotoxigenicPathogens (Basel, Switzerland) · 2020Review
- iRAPs curb antisense transcription in E. coli.Nucleic acids research · 2019Article
- SslE (YghJ), a Cell-Associated and Secreted Lipoprotein of Neonatal Septicemic Escherichia coli, Induces Toll-Like Receptor 2-Dependent Macrophage Activation and Proinflammation through NF-κB and MAP Kinase Signaling.Infection and immunity · 2018Article
- StpA and Hha stimulate pausing by RNA polymerase by promoting DNA-DNA bridging of H-NS filaments.Nucleic acids research · 2018Article
- secA, secD, secF, yajC, and yidC contribute to the adhesion regulation of Vibrio alginolyticus.MicrobiologyOpen · 2018Article
- Structure and Membrane Topography of the Vibrio-Type Secretin Complex from the Type 2 Secretion System of Enteropathogenic Escherichia coli.Journal of bacteriology · 2018Article
- Expanding Role of Type II Secretion in Bacterial Pathogenesis and Beyond.Infection and immunity · 2017Review
- Electron Acceptors Induce Secretion of Enterotoxigenic Escherichia coli Heat-Labile Enterotoxin under Anaerobic Conditions through Promotion of GspD Assembly.Infection and immunity · 2016Article
- Animal Enterotoxigenic Escherichia coli.EcoSal Plus · 2016Review
- Differential Regulation of the Surface-Exposed and Secreted SslE Lipoprotein in Extraintestinal Pathogenic Escherichia coli.PloS one · 2016Article
- Murine model of chemotherapy-induced extraintestinal pathogenic Escherichia coli translocation.Infection and immunity · 2015Article
- The type II secretion pathway in Vibrio cholerae is characterized by growth phase-dependent expression of exoprotein genes and is positively regulated by σE.Infection and immunity · 2014Article
- SslE elicits functional antibodies that impair in vitro mucinase activity and in vivo colonization by both intestinal and extraintestinal Escherichia coli strains.PLoS pathogens · 2014Article
- Control of bacterial virulence by the RalR regulator of the rabbit-specific enteropathogenic Escherichia coli strain E22.Infection and immunity · 2013Article
- The non-pathogenic Escherichia coli strain W secretes SslE via the virulence-associated type II secretion system beta.BMC microbiology · 2013Article
- RegR virulence regulon of rabbit-specific enteropathogenic Escherichia coli strain E22.Infection and immunity · 2013Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gene cluster gspCDEFGHIJKLM codes for various structural components of the type II secretion pathway which is responsible for the secretion of heat-labile enterotoxin by enterotoxigenic Escherichia coli (ETEC). In this work, we used a variety of molecular approaches to elucidate the transcriptional organization of the ETEC type II secretion system and to unravel the mechanisms by which the expression of these genes is controlled. We showed that the gspCDEFGHIJKLM cluster and three other upstream genes, yghJ, pppA, and yghG, are cotranscribed and that a promoter located in the upstream region of yghJ plays a major role in the expression of this 14-gene transcriptional unit. Transcription of the yghJ promoter was repressed 168-fold upon a temperature downshift from 37 degrees C to 22 degrees C. This temperature-induced repression was mediated by the global regulatory proteins H-NS and StpA. Deletion mutagenesis showed that the promoter region encompassing positions -321 to +301 relative to the start site of transcription of yghJ was required for full repression. The yghJ promoter region is predicted to be highly curved and bound H-NS or StpA directly. The binding of H-NS or StpA blocked transcription initiation by inhibiting promoter open complex formation. Unraveling the mechanisms of regulation of type II secretion by ETEC enhances our understanding of the pathogenesis of ETEC and other pathogenic varieties of E. coli.
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