ArticleJournal of bacteriology2007
Minimum length requirement of the flexible N-terminal translocation subdomain of colicin E3.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 14 citations in OpenAlex.
- Optimizing Cell-Free Protein Synthesis for Increased Yield and Activity of Colicins.Methods and protocols · 2019Article
- Rapid production and characterization of antimicrobial colicins usingSynthetic biology (Oxford, England) · 2018Article
- In vivo processing of DNase colicins E2 and E7 is required for their import into the cytoplasm of target cells.PloS one · 2014Article
- FtsH-dependent processing of RNase colicins D and E3 means that only the cytotoxic domains are imported into the cytoplasm.The Journal of biological chemistry · 2011Article
- Cloning, purification, and functional characterization of Carocin S2, a ribonuclease bacteriocin produced by Pectobacterium carotovorum.BMC microbiology · 2011Article
- Directed epitope delivery across the Escherichia coli outer membrane through the porin OmpF.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Unimolecular study of the interaction between the outer membrane protein OmpF from E. coli and an analogue of the HP(2-20) antimicrobial peptide.Journal of bioenergetics and biomembranes · 2010Article
- Genome-wide screens: novel mechanisms in colicin import and cytotoxicity.Molecular microbiology · 2009Article
- Energy-dependent immunity protein release during tol-dependent nuclease colicin translocation.The Journal of biological chemistry · 2009Article
- Investigating early events in receptor binding and translocation of colicin E9 using synchronized cell killing and proteolytic cleavage.Journal of bacteriology · 2008Article
- Colicin biology.Microbiology and molecular biology reviews : MMBR · 2007Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The 315-residue N-terminal T domain of colicin E3 functions in translocation of the colicin across the outer membrane through its interaction with outer membrane proteins including the OmpF porin. The first 83 residues of the T domain are known from structure studies to be disordered. This flexible translocation subdomain contains the TolB box (residues 34 to 46) that must cross the outer membrane in an early translocation event, allowing the colicin to bind to the TolB protein in the periplasm. In the present study, it was found that cytotoxicity of the colicin requires a minimum length of 19 to 23 residues between the C terminus (residue 46) of the TolB box and the end of the flexible subdomain (residue 83). Colicin E3 molecules of sufficient length display normal binding to TolB and occlusion of OmpF channels in vitro. The length of the N-terminal subdomain is critical because it allows the TolB box to cross the outer membrane and interact with TolB. It is proposed that the length constraint is a consequence of ordered structure in the downstream segment of the T domain (residues 84 to 315) that prevents its insertion through the outer membrane via a translocation pore that includes OmpF.
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Registered trials
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