ArticleBiophysical journal2007
Particle image correlation spectroscopy (PICS): retrieving nanometer-scale correlations from high-density single-molecule position data.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed.
- Analysis of the H-Ras mobility pattern in vivo shows cellular heterogeneity inside epidermal tissue.Disease models & mechanisms · 2022Article
- Analysis of intracellular protein dynamics in living zebrafish embryos using light-sheet fluorescence single-molecule microscopy.Biomedical optics express · 2021Article
- Review
- Ultrafast data mining of molecular assemblies in multiplexed high-density super-resolution images.Nature communications · 2019Article
- Estimation of diffusion constants from single molecular measurement without explicit tracking.BMC systems biology · 2018Article
- Correlation analysis framework for localization-based superresolution microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Impairment on the lateral mobility induced by structural changes underlies the functional deficiency of the lupus-associated polymorphism FcγRIIB-T232.The Journal of experimental medicine · 2016Article
- Development of an image Mean Square Displacement (iMSD)-based method as a novel approach to study the intracellular trafficking of nanoparticles.Acta biomaterialia · 2016Article
- Spatiotemporal Fluctuation Analysis: A Powerful Tool for the Future Nanoscopy of Molecular Processes.Biophysical journal · 2016Article
- Resolving Fast, Confined Diffusion in Bacteria with Image Correlation Spectroscopy.Biophysical journal · 2016Article
- An Intermittent Model for Intracellular Motions of Gold Nanostars by k-Space Scattering Image Correlation.Biophysical journal · 2015Article
- Imaging stress.Cell stress & chaperones · 2015Article
- Steady-state cross-correlations for live two-colour super-resolution localization data sets.Nature communications · 2015Article
- Dances with Membranes: Breakthroughs from Super-resolution Imaging.Current topics in membranes · 2015Review
- Depth-of-Focus Correction in Single-Molecule Data Allows Analysis of 3D Diffusion of the Glucocorticoid Receptor in the Nucleus.PloS one · 2015Article
- Imaging live cells at the nanometer-scale with single-molecule microscopy: obstacles and achievements in experiment optimization for microbiology.Molecules (Basel, Switzerland) · 2014Review
- Classification of dynamical diffusion states in single molecule tracking microscopy.Biophysical journal · 2014Article
- Easy measurement of diffusion coefficients of EGFP-tagged plasma membrane proteins using k-Space Image Correlation Spectroscopy.Journal of visualized experiments : JoVE · 2014Article
- Single-molecule detection and tracking in plants.Protoplasma · 2014Review
- Quantitation of glucocorticoid receptor DNA-binding dynamics by single-molecule microscopy and FRAP.PloS one · 2014Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new data analysis tool that resolves correlations on the nanometer length and millisecond timescale is derived. This tool, adapted from methods of spatiotemporal image correlation spectroscopy, exploits the high positional accuracy of single-particle tracking. While conventional tracking methods break down if multiple particle trajectories intersect, our method works in principle for arbitrarily large molecule densities and diffusion coefficients as long as individual molecules can be identified. The method is computationally cheap and robust and requires no a priori knowledge about the dynamical coefficients, as opposed to other methods. We demonstrate the validity of the method by Monte Carlo simulations and by application to single-molecule tracking data of membrane-anchored proteins in live cells. The results faithfully reproduce those obtained by conventional tracking. Upon activation, a fraction of the small GTPase H-Ras is confined to domains of <200 nm diameter, which further substantiates the prediction that membrane organization is a determinant in cellular signaling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.