ArticleBiophysical journal2007
Quantitative characterization of biological liquids for third-harmonic generation microscopy.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Third harmonic generation microscopy reveals structure and mucus dynamics in human airway epithelium models.iScience · 2026Article
- On the importance of simultaneous label-free multimodal nonlinear optical imaging for biomedical applications.APL photonics · 2025Article
- Color-resolved third harmonic generation microscopy for single-RBC HbA1c measurement and glycemic history assessment.Science advances · 2025Article
- Simultaneous label-free autofluorescence multi-harmonic microscopy driven by the supercontinuum generated from a bulk nonlinear crystal.Biomedical optics express · 2024Article
- Third harmonic imaging contrast from tubular structures in the presence of index discontinuity.Scientific reports · 2023Article
- Terahertz pump-probe of liquid water at 12.3 THz.Physical chemistry chemical physics : PCCP · 2022Article
- Compact portable multiphoton microscopy reveals histopathological hallmarks of unprocessed lung tumor tissue in real time.Translational biophotonics · 2020Article
- Towards Intense THz Spectroscopy on Water: Characterization of Optical Rectification by GaP, OH1, and DSTMS at OPA Wavelengths.Materials (Basel, Switzerland) · 2020Article
- Second and third harmonic generation microscopy visualizes key structural components in fresh unprocessed healthy human breast tissue.Journal of biophotonics · 2019Article
- Label-Free Automated Cell Tracking: Analysis of the Role of E-cadherin Expression in Collective Electrotaxis.Cellular and molecular bioengineering · 2017Article
- Third harmonic generation imaging for fast, label-free pathology of human brain tumors.Biomedical optics express · 2016Article
- Polarized THG microscopy identifies compositionally different lipid droplets in mammalian cells.Biophysical journal · 2014Article
- Multimodal nonlinear optical microscopic imaging provides new insights into acetowhitening mechanisms in live mammalian cells without labeling.Biomedical optics express · 2014Article
- Label-free imaging of lipid depositions in C. elegans using third-harmonic generation microscopy.PloS one · 2014Article
- Non-invasive monitoring of cell metabolism and lipid production in 3D engineered human adipose tissues using label-free multiphoton microscopy.Biomaterials · 2013Article
- Cross-phase modulation spectral shifting: nonlinear phase contrast in a pump-probe microscope.Biomedical optics express · 2012Article
- Intravital third harmonic generation microscopy of collective melanoma cell invasion: Principles of interface guidance and microvesicle dynamics.Intravital · 2012Article
- Combined third-harmonic generation and four-wave mixing microscopy of tissues and embryos.Biomedical optics express · 2011Article
- In vivo imaging of myelin in the vertebrate central nervous system using third harmonic generation microscopy.Biophysical journal · 2011Article
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2 authors.
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Abstract
Third-harmonic generation (THG) microscopy provides images of unstained biological samples based on spatial variations in third-order nonlinear susceptibility, refractive index, and dispersion. In this study, we establish quantitative values for the third-order nonlinear susceptibilities of several solvents (water, ethanol, glycerol), physiological aqueous (ions, amino acids, polypeptides, bovine serum albumin, glucose) and lipid (triglycerides, cholesterol) solutions as a function of solute concentration in the 1.05-1.25 microm excitation range. We use these data in conjunction with imaging experiments to show that THG imaging with approximately 1.2 microm excitation lacks specificity and sensitivity to detect physiological ion concentration changes, and that nonaqueous structures such as lipid bodies provide a more robust source of signal. Finally, we illustrate the impact of index-matching liquids in THG images. These data provide a basis for interpreting biological THG images and for developing additional applications.
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