ArticlePigment cell research2006
The Fugu tyrp1 promoter directs specific GFP expression in zebrafish: tools to study the RPE and the neural crest-derived melanophores.
Article in Pigment cell research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 46 citations in OpenAlex.
- A Dosage-Dependent Dominant-Negative Mechanism of SLC45A2(W74R) in Autosomal Dominant Oculocutaneous Albinism Revealed by Zebrafish Modeling.Translational vision science & technology · 2026Article
- Functional test of a naturally occurred tumor modifier gene provides insights to melanoma development.G3 (Bethesda, Md.) · 2025Article
- Stretching of the retinal pigment epithelium contributes to zebrafish optic cup morphogenesis.eLife · 2021Article
- Developmental chromatin programs determine oncogenic competence in melanoma.Science (New York, N.Y.) · 2021Article
- pH-controlled histone acetylation amplifies melanocyte differentiation downstream of MITF.EMBO reports · 2020Article
- Identification of myosin II as a cripto binding protein and regulator of cripto function in stem cells and tissue regeneration.Biochemical and biophysical research communications · 2019Article
- R-Fluoxetine Increases Melanin Synthesis Through a 5-HT1A/2A Receptor and p38 MAPK Signaling Pathways.International journal of molecular sciences · 2018Article
- Distant Insulin Signaling Regulates Vertebrate Pigmentation through the Sheddase Bace2.Developmental cell · 2018Article
- Expression patterns of zebrafishMolecular vision · 2017Article
- Development of a fluorescent transgenic zebrafish biosensor for sensing aquatic heavy metal pollution.Transgenic research · 2016Article
- Kit signaling is involved in melanocyte stem cell fate decisions in zebrafish embryos.Development (Cambridge, England) · 2013Article
- Development of translating ribosome affinity purification for zebrafish.Genesis (New York, N.Y. : 2000) · 2013Article
- A robust procedure for distinctively visualizing zebrafish retinal cell nuclei under bright field light microscopy.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2013Article
- Clonal and lineage analysis of melanocyte stem cells and their progeny in the zebrafish.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Differentiated melanocyte cell division occurs in vivo and is promoted by mutations in Mitf.Development (Cambridge, England) · 2011Article
- mitfa is required at multiple stages of melanocyte differentiation but not to establish the melanocyte stem cell.Developmental biology · 2011Article
- Lineage relationship of direct-developing melanocytes and melanocyte stem cells in the zebrafish.PloS one · 2011Article
- Clonal analyses reveal roles of organ founding stem cells, melanocyte stem cells and melanoblasts in establishment, growth and regeneration of the adult zebrafish fin.Development (Cambridge, England) · 2010Article
- Differential contribution of direct-developing and stem cell-derived melanocytes to the zebrafish larval pigment pattern.Developmental biology · 2010Article
- Stepwise maturation of apicobasal polarity of the neuroepithelium is essential for vertebrate neurulation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
In vertebrates, pigment cells account for a small percentage of the total cell population and they intermingle with other cell types. This makes it difficult to isolate them for analyzes of their functions in the context of development. To alleviate such difficulty, we generated two stable transgenic zebrafish lines (pt101 and pt102) that express green fluorescent protein (GFP) in melanophores under the control of the 1 kb Fugu tyrp1 promoter. In pt101, GFP is expressed in both retinal pigment epithelium (RPE) cells and the neural crest-derived melanophores (NCDM), whereas in pt102, GFP is predominately expressed in the NCDM. Our results indicate that the Fugu tyrp1 promoter can direct transgene expression in a cell-type-specific manner in zebrafish. In addition, our findings provide evidence supporting differential regulations of melanin-synthesizing genes in RPE cells and the NCDM in zebrafish. Utilizing the varying GFP expression levels in these fish, we have isolated melanophores via flow cytometry and revealed the capability of sorting the NCDM from RPE cells as well. Thus, these transgenic lines are useful tools to study melanophores in zebrafish.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.