ArticleThe EMBO journal2006
Reversible, cooperative reactions of yeast vacuole docking.
Article in The EMBO journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Phosphatidic acid induces conformational changes in Sec18 protomers that prevent SNARE priming.The Journal of biological chemistry · 2019Article
- Multivalent Rab interactions determine tether-mediated membrane fusion.Molecular biology of the cell · 2017Article
- The Central Polybasic Region of the Soluble SNARE (Soluble N-Ethylmaleimide-sensitive Factor Attachment Protein Receptor) Vam7 Affects Binding to Phosphatidylinositol 3-Phosphate by the PX (Phox Homology) Domain.The Journal of biological chemistry · 2016Article
- SNAREs support atlastin-mediated homotypic ER fusion in Saccharomyces cerevisiae.The Journal of cell biology · 2015Article
- In vitro assay using engineered yeast vacuoles for neuronal SNARE-mediated membrane fusion.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Fusion proteins and select lipids cooperate as membrane receptors for the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) Vam7p.The Journal of biological chemistry · 2013Article
- Astrocytes release D-serine by a large vesicle.Neuroscience · 2013Article
- Two distinct modes of exocytotic fusion pore expansion in large astrocytic vesicles.The Journal of biological chemistry · 2013Article
- LegC3, an effector protein from Legionella pneumophila, inhibits homotypic yeast vacuole fusion in vivo and in vitro.PloS one · 2013Article
- Sec1/Munc18 protein Vps33 binds to SNARE domains and the quaternary SNARE complex.Molecular biology of the cell · 2012Article
- Yeast lipin 1 orthologue pah1p regulates vacuole homeostasis and membrane fusion.The Journal of biological chemistry · 2012Article
- Requirement for Golgi-localized PI(4)P in fusion of COPII vesicles with Golgi compartments.Molecular biology of the cell · 2011Article
- Reconstituted membrane fusion requires regulatory lipids, SNAREs and synergistic SNARE chaperones.The EMBO journal · 2008Article
- Membrane fusion.Nature structural & molecular biology · 2008Review
- HOPS proofreads the trans-SNARE complex for yeast vacuole fusion.Molecular biology of the cell · 2008Article
- Sec18p and Vam7p remodel trans-SNARE complexes to permit a lipid-anchored R-SNARE to support yeast vacuole fusion.The EMBO journal · 2007Article
- Excess vacuolar SNAREs drive lysis and Rab bypass fusion.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Assays of vacuole fusion resolve the stages of docking, lipid mixing, and content mixing.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Trans-SNARE complex assembly and yeast vacuole membrane fusion.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Homotypic yeast vacuole fusion occurs in three stages: (i) priming reactions, which are independent of vacuole clustering, (ii) docking, in which vacuoles cluster and accumulate fusion proteins and fusion regulatory lipids at a ring-shaped microdomain surrounding the apposed membranes of each docked vacuole, where fusion will occur, and (iii) bilayer fusion/compartment mixing. These stages require vacuolar SNAREs, SNARE-chaperones, GTPases, effector complexes, and chemically minor but functionally important lipids. For each, we have developed specific ligands that block fusion and conditions that reverse each block. Using them, we test whether docking entails a linearly ordered series of catalytic events, marked by sequential acquisition of resistance to inhibitors, or whether docking subreactions are cooperative and/or reversible. We find that each fusion protein and regulatory lipid is needed throughout docking, indicative of a reversible or highly cooperative assembly of the fusion-competent vertex ring. In accord with this cooperativity, vertices enriched in one fusion catalyst are enriched in others. Docked vacuoles finally assemble SNARE complexes, yet still require physiological temperature and lipid rearrangements to complete fusion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.