ArticleProceedings of the National Academy of Sciences of the United States of America2006
Multiple pathways of selected gene amplification during adaptive mutation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 131 citations in OpenAlex.
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- Inter-Relationship Between a Transcriptional Regulator of Flagella GenesBioMed research international · 2022Article
- Evolution of new enzymes by gene duplication and divergence.The FEBS journal · 2020Review
- Selective Inbreeding: Genetic Crosses Drive Apparent Adaptive Mutation in the Cairns-Foster System ofGenetics · 2020Article
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- Experimental Evolution of Innovation and Novelty.Trends in ecology & evolution · 2019Review
- Selection-Enhanced Mutagenesis ofGenetics · 2018Article
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- Competing for Iron: Duplication and Amplification of the isd Locus in Staphylococcus lugdunensis HKU09-01 Provides a Competitive Advantage to Overcome Nutritional Limitation.PLoS genetics · 2016Article
- Host Mitochondrial Association Evolved in the Human Parasite Toxoplasma gondii via Neofunctionalization of a Gene Duplicate.Genetics · 2016Article
- Reinterpreting Long-Term Evolution Experiments: Is Delayed Adaptation an Example of Historical Contingency or a Consequence of Intermittent Selection?Journal of bacteriology · 2016Article
- Rapid Evolution of Citrate Utilization by Escherichia coli by Direct Selection Requires citT and dctA.Journal of bacteriology · 2016Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
In a phenomenon referred to as "adaptive mutation," a population of bacterial cells with a mutation in the lac operon (lac-) accumulates Lac+ revertants during prolonged exposure to selective growth conditions (lactose). Evidence was provided that selective conditions do not increase the mutation rate but instead favor the growth of rare cells with a duplication of the leaky lac allele. A further increase in copy number (amplification) improves growth and increases the likelihood of a sequence change by adding more mutational targets to the clone (cells and lac copies per cell). These duplications and amplifications are described here. Before selection, cells with large (134-kb) lac duplications and long junction sequences (>1 kb) were common (0.2%). The same large repeats were found after selection in cells with a low-copy-number lac amplification. Surprisingly, smaller repeats (average, 34 kb) were found in high-copy-number amplifications. The small-repeat duplications form when deletions modify a preexisting large-repeat duplication. The shorter repeat size allowed higher lac amplification and better growth on lactose. Thus, selection favors a succession of gene-amplification types that make sequence changes more probable by adding targets. These findings are relevant to genetic adaptation in any biological systems in which fitness can be increased by adding gene copies (e.g., cancer and bacterial drug resistance).
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