ArticleProteomics2006
Proteomic analysis of reporter genes for molecular imaging of transplanted embryonic stem cells.
Article in Proteomics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 48 citations in OpenAlex.
- In vivo observation of multi-phase spatiotemporal cellular dynamics of transplanted HSPCs during early engraftment.FASEB bioAdvances · 2022Article
- Endothelial colony forming cells and mesenchymal progenitor cells form blood vessels and increase blood flow in ischemic muscle.Scientific reports · 2017Article
- Increased Understanding of Stem Cell Behavior in Neurodegenerative and Neuromuscular Disorders by Use of Noninvasive Cell Imaging.Stem cells international · 2016Review
- Stem Cell Imaging: Tools to Improve Cell Delivery and Viability.Stem cells international · 2016Review
- (124)I-PET Assessment of Human Sodium Iodide Symporter Reporter Gene Activity for Highly Sensitive In Vivo Monitoring of Teratoma Formation in Mice.Molecular imaging and biology · 2015Article
- Molecular engineering of nonmetallic biosensors for CEST MRI.ACS chemical biology · 2015Article
- Human placenta mesenchymal stem cells expressing exogenous kringle1-5 protein by fiber-modified adenovirus suppress angiogenesis.Cancer gene therapy · 2014Article
- Article
- Human vasculogenic cells form functional blood vessels and mitigate adverse remodeling after ischemia reperfusion injury in rats.Angiogenesis · 2013Article
- Molecular imaging: the key to advancing cardiac stem cell therapy.Trends in cardiovascular medicine · 2013Review
- In vivo imaging of embryonic stem cell therapy.European journal of nuclear medicine and molecular imaging · 2011Review
- Cardiovascular molecular imaging: focus on clinical translation.Circulation · 2011Review
- Imaging of embryonic stem cell migration in vivo.Methods in molecular biology (Clifton, N.J.) · 2011Article
- Tumorigenicity of pluripotent stem cells: biological insights from molecular imaging.Journal of the Royal Society, Interface · 2010Review
- Methods to assess stem cell lineage, fate and function.Advanced drug delivery reviews · 2010Review
- Role of molecular imaging in stem cell therapy for myocardial restoration.Trends in cardiovascular medicine · 2010Review
- Embryonic stem cell-derived endothelial cells engraft into the ischemic hindlimb and restore perfusion.Arteriosclerosis, thrombosis, and vascular biology · 2010Article
- In vivo imaging and monitoring of transplanted stem cells: clinical applications.Current cardiology reports · 2010Review
- Effects of cell number on teratoma formation by human embryonic stem cells.Cell cycle (Georgetown, Tex.) · 2009Article
- Long term non-invasive imaging of embryonic stem cells using reporter genes.Nature protocols · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
Study of stem cells may reveal promising treatment for diseases. The fate and function of transplanted stem cells remain poorly defined. Recent studies demonstrate that reporter genes can monitor real-time survival of transplanted stem cells in living subjects. We examined the effects of a novel and versatile triple fusion (TF) reporter gene construction on embryonic stem (ES) cell function by proteomic analysis. Murine ES cells were stably transduced with a self-inactivating lentiviral vector containing fluorescence (firefly luciferase; Fluc), bioluminescence (monomeric red fluorescence protein; mRFP), and positron emission tomography (herpes simplex virus type 1 truncated thymidine kinase; tTK) reporter genes. Fluorescence-activated cell sorting (FACS) analysis isolated stably transduced populations. TF reporter gene effects on cellular function were evaluated by quantitative proteomic profiling of control ES cells versus ES cells stably expressing the TF construct (ES-TF). Overall, no significant changes in protein quantity were observed. TF reporter gene expression had no effect on ES cell viability, proliferation, and differentiation capability. Molecular imaging studies tracked ES-TF cell survival and proliferation in living animals. In summary, this is the first proteomic study, demonstrating the unique potential of reporter gene imaging for tracking ES cell transplantation non-invasively, repetitively, and quantitatively.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.