ArticleMolecular biology of the cell2007
Akt-PDK1 complex mediates epidermal growth factor-induced membrane protrusion through Ral activation.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 65 citations in OpenAlex.
- Spatiotemporal and quantitative analyses of phosphoinositides - fluorescent probe-and mass spectrometry-based approaches.FEBS letters · 2026Review
- The multifaceted significance of phosphoinositides in endocytic trafficking.FEBS letters · 2026Review
- Lysosomal PIPProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Multiphoton intravital microscopy of rodents.Nature reviews. Methods primers · 2022Article
- Article
- TROP2 increases growth and metastasis of human oral squamous cell carcinoma through activation of the PI3K/Akt signaling pathway.International journal of molecular medicine · 2019Article
- Upregulation of multiple signaling pathways by Dock5 deletion in epithelial cells.Molecular vision · 2017Article
- Article
- Allosteric substrate switching in a voltage-sensing lipid phosphatase.Nature chemical biology · 2016Article
- Fluorescent proteins as genetically encoded FRET biosensors in life sciences.Sensors (Basel, Switzerland) · 2015Review
- An optogenetic system for interrogating the temporal dynamics of Akt.Scientific reports · 2015Article
- Akt signaling dynamics in individual cells.Journal of cell science · 2015Article
- Polyphosphoinositide binding domains: Key to inositol lipid biology.Biochimica et biophysica acta · 2015Review
- A method to control phosphoinositides and to analyze PTEN function in living cells using voltage sensitive phosphatases.Frontiers in pharmacology · 2015Article
- Developments in preclinical cancer imaging: innovating the discovery of therapeutics.Nature reviews. Cancer · 2014Review
- Genetically encoded fluorescent biosensors for live-cell visualization of protein phosphorylation.Chemistry & biology · 2014Review
- Spatiotemporal dynamics of phosphorylation in lipid second messenger signaling.Molecular & cellular proteomics : MCP · 2013Review
- RCP-driven α5β1 recycling suppresses Rac and promotes RhoA activity via the RacGAP1-IQGAP1 complex.The Journal of cell biology · 2013Article
- Fluorescence resonance energy transfer microscopy as demonstrated by measuring the activation of the serine/threonine kinase Akt.Nature protocols · 2013Article
- The high and low molecular weight forms of hyaluronan have distinct effects on CD44 clustering.The Journal of biological chemistry · 2012Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We studied the spatiotemporal regulation of Akt (also called protein kinase B), phosphatidylinositol-3,4-bisphosphate [PtdIns(3,4)P2], and phosphatidylinositol-3,4,5-trisphosphate [PtdIns(3,4,5)P3] by using probes based on the principle of fluorescence resonance energy transfer. On epidermal growth factor (EGF) stimulation, the amount of PtdIns(3,4,5)P3 was increased diffusely in the plasma membrane, whereas that of PtdIns(3,4)P2 was increased more in the nascent lamellipodia than in the plasma membrane of the central region. The distribution and time course of Akt activation were similar to that of increased PtdIns(3,4)P2 levels, which were most prominent in the nascent lamellipodia. Moreover, we found that upon EGF stimulation 3-phosphoinositide-dependent protein kinase-1 (PDK1) was also recruited to nascent lamellipodia in an Akt-dependent manner. Because PDK1 is known to activate Ral GTPase and because Ral is required for EGF-induced lamellipodial protrusion, we speculated that the PDK1-Akt complex may be indispensable for the induction of lamellipodia. In agreement with this idea, EGF-induced lamellipodia formation was promoted by the overexpression of Akt and inhibited by an Akt inhibitor or a Ral-binding domain of Sec5. These results identified the Akt-PDK1 complex as an upstream positive regulator of Ral GTPase in the induction of lamellipodial protrusion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.