ArticleThe Journal of physiology1990
The response induced by intracellular cyclic AMP in isolated olfactory receptor cells of the newt.
Article in The Journal of physiology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 142 citations in OpenAlex.
- Shedding light on human olfaction: Electrophysiological recordings from sensory neurons in acute slices of olfactory epithelium.iScience · 2023Article
- Segregation of Ca2+ signaling in olfactory signal transduction.The Journal of general physiology · 2023Article
- Second messenger molecules have a limited spread in olfactory cilia.The Journal of general physiology · 2018Article
- Diverse Regulation of Temperature Sensation by Trimeric G-Protein Signaling in Caenorhabditis elegans.PloS one · 2016Article
- Potential role of transient receptor potential channel M5 in sensing putative pheromones in mouse olfactory sensory neurons.PloS one · 2013Article
- Distribution, amplification, and summation of cyclic nucleotide sensitivities within single olfactory sensory cilia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Mechanism of signal amplification in the olfactory sensory cilia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Modulation of the olfactory CNG channel by Ptdlns(3,4,5)P3.The Journal of membrane biology · 2004Article
- Contribution of cyclic-nucleotide-gated channels to the resting conductance of olfactory receptor neurons.Biophysical journal · 2003Article
- Olfactory signal transduction in the mouse septal organ.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Photolysis of caged cyclic AMP in the ciliary cytoplasm of the newt olfactory receptor cell.The Journal of physiology · 2002Article
- Responses to prolonged odour stimulation in frog olfactory receptor cells.The Journal of physiology · 2001Article
- Molecular determinants of the modulation of cyclic nucleotide-activated channels by calmodulin.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Na+-dependent Ca2+ extrusion governs response recovery in frog olfactory receptor cells.The Journal of general physiology · 1998Article
- Imaging odor-induced calcium transients in single olfactory cilia: specificity of activation and role in transduction.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Modulation of GABAC response by Ca2+ and other divalent cations in horizontal cells of the catfish retina.The Journal of general physiology · 1997Article
- Calcium entry through cyclic nucleotide-gated channels in individual cilia of olfactory receptor cells: spatiotemporal dynamics.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- Electrophysiological characterization of chemosensory neurons from the mouse vomeronasal organ.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1996Article
- Gated conductances in native and reconstituted membranes from frog olfactory cilia.Biophysical journal · 1996Article
- Olfactory transduction is intrinsically noisy.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Responses induced by intracellular cyclic nucleotides were analysed in isolated olfactory receptor cells of the newt under a voltage-clamp condition by using the patch pipette in a whole-cell recording configuration. Cyclic nucleotides were applied by diffusion from the patch pipette. 2. Introduction of either cyclic AMP or cyclic GMP caused a transient inward current in cells held at -50 mV. The response amplitude was dose-dependent with the Hill coefficient of 3 and half-saturating concentration of 300 microM (concentration in the pipette) for both cyclic AMP and cyclic GMP. Cyclic CMP was less effective than those two nucleotides. 3. The response to intracellular cyclic AMP was seen in all cilia-bearing cells, but not in cells which lost the cilia during dissociation. The response latency was shorter when cyclic AMP was introduced into the ciliated terminal swelling (ca 0.2 s) rather than into the cell body (ca 1.4 s). These results suggest that the sensitivity to intracellular cyclic AMP is confined to the cilia. 4. The cyclic AMP-induced current was transient (half decay time, ca 2.3s) despite the fact that cyclic AMP was continuously loaded from the patch pipette. The response time course was controlled by Ca2+; the reduction of external Ca2+ concentration (replaced with Mg2+) or loading the cell with 50 mM-EGTA prolonged the cyclic AMP-induced responses. The Ca2(+)-induced suppression was reversible. 5. The reversal potential of the cyclic AMP-induced transient current was -4.8 +/- 3.8 mV, and that of the current re-induced by Ca2+ removal was 1.5 +/- 2.1 mV, suggesting that both currents flowed through the same ionic channel. The channel permeates all alkali metal ions with the permeability ratios of PLi:PNa:PK:PRb:PCs = 0.93:1:0.93:0.91:0.72, but not Cl- or choline ions. 6. These results demonstrate that the cyclic AMP-induced response and the odorant-induced response of the isolated olfactory cell have nearly identical characteristics. The present study supports the notion that cyclic AMP is the internal messenger mediating olfactory transduction.
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