ArticleJournal of virology2007
Visualization of the interaction between the precursors of VPg, the viral protein linked to the genome of turnip mosaic virus, and the translation eukaryotic initiation factor iso 4E in Planta.
Article in Journal of virology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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Who cites it
79 citing papers in PubMed, 165 citations in OpenAlex.
- Turnip mosaic virus utilizes the lipid droplet biogenesis machinery to facilitate its propagation in plants.The New phytologist · 2026Article
- Molecular gatekeepers: eukaryotic translation factors decoding plant-virus dynamics for resistance engineering.Stress biology · 2026Review
- Interaction Between Translation Initiation Factor eIF4E in Banana and the VPg Protein of Banana Bract Mosaic Virus Reveals Potential Targets for Genome Editing.Molecular biotechnology · 2025Article
- Simultaneous knockout of multipleaBIOTECH · 2025Article
- Turnip mosaic virus infection cleaves MEDIATOR SUBUNIT16 in plants increasing plant susceptibility to the virus and its aphid vector Myzus persicae.BMC plant biology · 2025Article
- The RING-type E3 ligase RIE1 sustains leaf longevity by specifically targeting AtACS7 to fine-tune ethylene production inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- The 6-kilodalton peptide 1 in plant viruses of the family Potyviridae is a viroporin.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- The mystery remains: How do potyviruses move within and between cells?Molecular plant pathology · 2023Review
- Soybean 40S Ribosomal Protein S8 (GmRPS8) Interacts with 6K1 Protein and Contributes to Soybean Susceptibility toViruses · 2023Article
- CRISPR/Cas9-mediated gene editing to confer turnip mosaic virus (TuMV) resistance in Chinese cabbage (Horticulture research · 2023Article
- Article
- Plant eIF4E isoforms as factors of susceptibility and resistance to potyviruses.Frontiers in plant science · 2023Review
- Effector-mediated plant-virus-vector interactions.The Plant cell · 2022Review
- StREM1.3 REMORIN Protein Plays an Agonistic Role in Potyvirus Cell-to-Cell Movement inViruses · 2022Article
- Proteolytic Processing of Plant Proteins by Potyvirus NIa Proteases.Journal of virology · 2022Article
- Protein Nucleotidylylation in +ssRNA Viruses.Viruses · 2021Review
- Sugarcane mosaic virus remodels multiple intracellular organelles to form genomic RNA replication sites.Archives of virology · 2021Article
- Review
- DEAD-box helicases modulate dicing body formation inScience advances · 2021Article
- Viral silencing suppressors and cellular proteins partner with plant RRP6-like exoribonucleases.Virus genes · 2020Article
19 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The RNA genome of Turnip mosaic virus is covalently linked at its 5' end to a viral protein known as VPg. This protein binds to the translation eukaryotic initiation factor iso 4E [eIF(iso)4E]. This interaction has been shown to be important for virus infection, although its exact biological function(s) has not been elucidated. In this study, we investigated the subcellular site of the VPg-eIF(iso)4E interaction using bimolecular fluorescence complementation (BiFC). As a first step, eIF(iso)4E, 6K-VPg-Pro, and VPg-Pro were expressed as full-length green fluorescent protein (GFP) fusions in Nicotiana benthamiana, and their subcellular localizations were visualized by confocal microscopy. eIF(iso)4E was predominantly associated with the endoplasmic reticulum (ER), and VPg-Pro was observed in the nucleus and possibly the nucleolus, while 6K-VPg-Pro-GFP induced the formation of cytoplasmic vesicles budding from the ER. In BiFC experiments, reconstituted green fluorescence was observed throughout the nucleus, with a preferential accumulation in subnuclear structures when the GFP split fragments were fused to VPg-Pro and eIF(iso)4E. On the other hand, the interaction of 6K-VPg-Pro with eIF(iso)4E was observed in cytoplasmic vesicles embedded in the ER. These data suggest that the association of VPg with the translation factor might be needed for two different functions, depending of the VPg precursor involved in the interaction. VPg-Pro interaction with eIF(iso)4E may be involved in perturbing normal cellular functions, while 6K-VPg-Pro interaction with the translation factor may be needed for viral RNA translation and/or replication.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.