ArticleJournal of molecular biology2007
Characteristics affecting expression and solubilization of yeast membrane proteins.
Article in Journal of molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 44 citations in OpenAlex.
- A 10-year meta-analysis of membrane protein structural biology: Detergents, membrane mimetics, and structure determination techniques.Biochimica et biophysica acta. Biomembranes · 2021Pooled it
- Refolding and characterization of two G protein-coupled receptors purified from E. coli inclusion bodies.PloS one · 2021Article
- Growth Inhibition by Amino Acids inMicroorganisms · 2020Article
- Mutant power: using mutant allele collections for yeast functional genomics.Briefings in functional genomics · 2016Review
- Identification of destabilizing and stabilizing mutations of Ste2p, a G protein-coupled receptor in Saccharomyces cerevisiae.Biochemistry · 2015Article
- Sparse and incomplete factorial matrices to screen membrane protein 2D crystallization.Journal of structural biology · 2015Article
- TiO₂-Mediated Photocatalytic Mineralization of a Non-Ionic Detergent: Comparison and Combination with Other Advanced Oxidation Procedures.Materials (Basel, Switzerland) · 2015Article
- Overexpressing human membrane proteins in stably transfected and clonal human embryonic kidney 293S cells.Nature protocols · 2012Article
- Photoaffinity labeling of Ras converting enzyme using peptide substrates that incorporate benzoylphenylalanine (Bpa) residues: improved labeling and structural implications.Bioorganic & medicinal chemistry · 2011Article
- Recombinant expression screening of P. aeruginosa bacterial inner membrane proteins.BMC biotechnology · 2010Article
- N-terminal domain of the V-ATPase a2-subunit displays integral membrane protein properties.Protein science : a publication of the Protein Society · 2010Article
- Purification of transmembrane proteins from Saccharomyces cerevisiae for X-ray crystallography.Protein expression and purification · 2010Article
- Active machine learning for transmembrane helix prediction.BMC bioinformatics · 2010Article
- Effective critical micellar concentration of a zwitterionic detergent: a fluorimetric study on n-dodecyl phosphocholine.Journal of fluorescence · 2010Article
- Tuning microbial hosts for membrane protein production.Microbial cell factories · 2009Review
- Ratiocinative screen of eukaryotic integral membrane protein expression and solubilization for structure determination.Journal of structural and functional genomics · 2009Review
- Selecting optimum eukaryotic integral membrane proteins for structure determination by rapid expression and solubilization screening.Journal of molecular biology · 2009Article
- Quality control of overexpressed membrane proteins.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Expression screening of integral membrane proteins from Helicobacter pylori 26695.Protein science : a publication of the Protein Society · 2007Article
- High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Biochemical and structural analysis of membrane proteins often critically depends on the ability to overexpress and solubilize them. To identify properties of eukaryotic membrane proteins that may be predictive of successful overexpression, we analyzed expression levels of the genomic complement of over 1000 predicted membrane proteins in a recently completed Saccharomyces cerevisiae protein expression library. We detected statistically significant positive and negative correlations between high membrane protein expression and protein properties such as size, overall hydrophobicity, number of transmembrane helices, and amino acid composition of transmembrane segments. Although expression levels of membrane and soluble proteins exhibited similar negative correlations with overall hydrophobicity, high-level membrane protein expression was positively correlated with the hydrophobicity of predicted transmembrane segments. To further characterize yeast membrane proteins as potential targets for structure determination, we tested the solubility of 122 of the highest expressed yeast membrane proteins in six commonly used detergents. Almost all the proteins tested could be solubilized using a small number of detergents. Solubility in some detergents depended on protein size, number of transmembrane segments, and hydrophobicity of predicted transmembrane segments. These results suggest that bioinformatic approaches may be capable of identifying membrane proteins that are most amenable to overexpression and detergent solubilization for structural and biochemical analyses. Bioinformatic approaches could also be used in the redesign of proteins that are not intrinsically well-adapted to such studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.