ArticleBreast cancer research : BCR2006
GATA3 protein as a MUC1 transcriptional regulator in breast cancer cells.
Article in Breast cancer research : BCR, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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16 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.
- Meta-analysis of human cancer microarrays reveals GATA3 is integral to the estrogen receptor alpha pathway.Molecular cancer · 2008Pooled it
- Hypoxia induces downregulation of the tumor-suppressive sST2 in colorectal cancer cells via the HIF-nuclear IL-33-GATA3 pathway.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Upregulated GATA3/miR205-5p Axis Inhibits MFNG Transcription and Reduces the Malignancy of Triple-Negative Breast Cancer.Cancers · 2022Article
- Melatonin Modulation of Radiation-Induced Molecular Changes in MCF-7 Human Breast Cancer Cells.Biomedicines · 2022Article
- Article
- MUC1-mediated motility in breast cancer: a review highlighting the role of the MUC1/ICAM-1/Src signaling triad.Clinical & experimental metastasis · 2015Review
- Cooperation of DLC1 and CDK6 affects breast cancer clinical outcome.G3 (Bethesda, Md.) · 2014Article
- Shift in GATA3 functions, and GATA3 mutations, control progression and clinical presentation in breast cancer.Breast cancer research : BCR · 2014Article
- GATA5 CpG island hypermethylation is an independent predictor for poor clinical outcome in renal cell carcinoma.Oncology reports · 2014Article
- Differential network analysis applied to preoperative breast cancer chemotherapy response.PloS one · 2013Article
- Suppression of IL-8 production in gastric epithelial cells by MUC1 mucin and peroxisome proliferator-associated receptor-γ.American journal of physiology. Gastrointestinal and liver physiology · 2012Article
- The role of tumor hypoxia in MUC1-positive breast carcinomas.Virchows Archiv : an international journal of pathology · 2011Article
- Breast cancer biomarker discovery in the functional genomic age: a systematic review of 42 gene expression signatures.Biomarker insights · 2010Article
- Influence of sublethal total-body irradiation on immune cell populations in the intestinal mucosa.Radiation research · 2010Article
- GATA3 in development and cancer differentiation: cells GATA have it!Journal of cellular physiology · 2010Review
- Gene microarray analysis of human renal cell carcinoma: the effects of HDAC inhibition and retinoid treatment.Cancer biology & therapy · 2008Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
introductionRecent studies have demonstrated that members of the GATA-binding protein (GATA) family (GATA4 and GATA5) might have pivotal roles in the transcriptional upregulation of mucin genes (MUC2, MUC3 and MUC4) in gastrointestinal epithelium. The zinc-finger GATA3 transcription factor has been reported to be involved in the growth control and differentiation of breast epithelial cells. In SAGE (serial analysis of gene expression) studies we observed an intriguing significant correlation between GATA3 and MUC1 mRNA expression in breast carcinomas. We therefore designed the present study to elucidate whether MUC1 expression is regulated by GATA3 in breast cancer cells.
methodsPromoter sequence analysis of the MUC1 gene identified six GATA cis consensus elements in the 5' flanking region (GATA1, GATA3 and four GATA-like sequences). Chromatin immunoprecipitation and electrophoretic mobility-shift assays were employed to study the presence of a functional GATA3-binding site. GATA3 and MUC1 expression was analyzed in vitro with a GATA3 knockdown assay. Furthermore, expression of GATA3 and MUC1 genes was analyzed by real-time RT-PCR and immunohistochemistry on breast cancer-specific tissue microarrays.
resultsWe confirmed the presence of a functional GATA3-binding site on the MUC1 promoter region in the MCF7 cell line. We determined that GATA3 knockdown assays led to a decrease in MUC1 protein expression in MCF7 and T47D cells. In addition, we detected a statistically significant correlation in expression between GATA3 and MUC1 genes at the mRNA and protein levels both in normal breast epithelium and in breast carcinomas (p = 0.01). GATA3 expression was also highly associated with estrogen receptor and progesterone receptor status (p = 0.0001) and tumor grade (p = 0.004) in breast carcinomas.
conclusionOur study provides evidence indicating that GATA3 is probably a mediator for the transcriptional upregulation of MUC1 expression in some breast cancers.
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