ArticleWorld journal of gastroenterology2006
Immunoproteome analysis of soluble and membrane proteins of Shigella flexneri 2457T.
Article in World journal of gastroenterology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed, 22 citations in OpenAlex.
- Identification and evaluation of novel vaccine candidates against Shigella flexneri through reverse vaccinology approach.Applied microbiology and biotechnology · 2021Article
- Reverse vaccinology approach for the identification and characterization of outer membrane proteins ofClinical and experimental vaccine research · 2020Article
- The periplasmic enzyme, AnsB, of Shigella flexneri modulates bacterial adherence to host epithelial cells.PloS one · 2014Article
- In vivo versus in vitro protein abundance analysis of Shigella dysenteriae type 1 reveals changes in the expression of proteins involved in virulence, stress and energy metabolism.BMC microbiology · 2011Article
- Outer membrane protein A (OmpA) of Shigella flexneri 2a, induces protective immune response in a mouse model.PloS one · 2011Article
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Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo profile the immunogenic proteins of Shigella flexneri (S. flexneri) expressed during human infection using a proteomic approach.
methodsSoluble and membrane protein extractions of S. flexneri 2457T were separated by two-dimensional gel electrophoresis (2-DE). Proteins were transferred to PVDF membrane and immunoblotted with sera from shigellosis patients. Reactive protein spots were matched to Coomassie stained gels run in parallel, cut out and trypsin digested. Matrix-assisted laser desorption/ionization time of flight-mass spectrometry (MALDI-TOF-MS) was used to determine the peptide mass fingerprints, which were searched in the MASCOT database to identify the protein.
resultsA total of 8 immunoreactive proteins were successfully identified from the Coomassie stained gels in three repeats. Six of these proteins have not previously been reported as immunogenic in S. flexneri. These proteins could be potential candidates for vaccine or attenuation studies.
conclusionSoluble and membrane proteins of S. flexneri 2457T have been screened by 2-DE and immunoblotting with sera from shigellosis patients. Eight proteins are identified as immunogenic.
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