ArticleInfection and immunity2007
In vivo gene expression analysis identifies genes required for enhanced colonization of the mouse urinary tract by uropathogenic Escherichia coli strain CFT073 dsdA.
Article in Infection and immunity, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 85 citations in OpenAlex.
- Bacterial resistance to leucyl-tRNA synthetase inhibitor GSK2251052 develops during treatment of complicated urinary tract infections.Antimicrobial agents and chemotherapy · 2015Trial
- D-Serine reduces the expression of the cytopathic genotoxin colibactin.Microbial cell (Graz, Austria) · 2023Article
- Glycosuria Alters Uropathogenic Escherichia coli Global Gene Expression and Virulence.mSphere · 2022Article
- The RyfA small RNA regulates oxidative and osmotic stress responses and virulence in uropathogenic Escherichia coli.PLoS pathogens · 2021Article
- Genomic characterization of fourJournal of oral microbiology · 2021Article
- Escherichia coli CFT073 Fitness Factors during Urinary Tract Infection: Identification Using an Ordered Transposon Library.Applied and environmental microbiology · 2020Article
- Malodorous biogenic amines in Escherichia coli-caused urinary tract infections in women-a metabolomics approach.Scientific reports · 2020Article
- GadE regulatesWorld journal of clinical infectious diseases · 2020Article
- Context-Dependent Requirements for FimH and Other Canonical Virulence Factors in Gut Colonization by Extraintestinal Pathogenic Escherichia coli.Infection and immunity · 2018Article
- Article
- Uropathogenic Escherichia coli-Associated Exotoxins.Microbiology spectrum · 2016Review
- Measuring Escherichia coli Gene Expression during Human Urinary Tract Infections.Pathogens (Basel, Switzerland) · 2016Article
- A Highly Conserved Bacterial D-Serine Uptake System Links Host Metabolism and Virulence.PLoS pathogens · 2016Article
- Genome-wide analysis of the response to nitric oxide in uropathogenicMicrobial genomics · 2015Article
- Virulence and Fitness Determinants of Uropathogenic Escherichia coli.Microbiology spectrum · 2015Review
- Metabolism and Fitness of Urinary Tract Pathogens.Microbiology spectrum · 2015Article
- Impact of microfluidic processing on bacterial ribonucleic acid expression.Biomicrofluidics · 2015Article
- dsdA Does Not Affect Colonization of the Murine Urinary Tract by Escherichia coli CFT073.PloS one · 2015Article
- From ingestion to colonization: the influence of the host environment on regulation of the LEE encoded type III secretion system in enterohaemorrhagic Escherichia coli.Frontiers in microbiology · 2015Review
- Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Deletional inactivation of the gene encoding d-serine deaminase, dsdA, in uropathogenic Escherichia coli strain CFT073 results in a hypermotile strain with a hypercolonization phenotype in the bladder and kidneys of mice in a model of urinary tract infection (UTI). The in vivo gene expression profiles of CFT073 and CFT073 dsdA were compared by isolating RNA directly from the urine of mice challenged with each strain individually. Hybridization of cDNAs derived from these samples to CFT073-specific microarrays allowed identification of genes that were up- or down-regulated in the dsdA deletion strain during UTI. Up-regulated genes included the known d-serine-responsive gene dsdX, suggesting in vivo intracellular accumulation of d-serine by CFT073 dsdA. Genes encoding F1C fimbriae, both copies of P fimbriae, hemolysin, OmpF, a dipeptide transporter DppA, a heat shock chaperone IbpB, and clusters of open reading frames with unknown functions were also up-regulated. To determine the role of these genes as well as motility in the hypercolonization phenotype, mutants were constructed in the CFT073 dsdA background and tested in competition against the wild type in the murine model of UTI. Strains with deletions of one or both of the two P fimbrial operons, hlyA, fliC, ibpB, c0468, locus c3566 to c3568, or c2485 to c2490 colonized mouse bladders and kidneys at levels indistinguishable from wild type. CFT073 dsdA c2398 and CFT073 dsdA focA maintained a hypercolonization phenotype. A CFT073 dsdA dppA mutant was attenuated 10- to 50-fold in its colonization ability compared to CFT073. Our results support a role for d-serine catabolism and signaling in global virulence gene regulation of uropathogenic E. coli.
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