ArticleYeast (Chichester, England)2006
Simplified primer design for PCR-based gene targeting and microarray primer database: two web tools for fission yeast.
Article in Yeast (Chichester, England), 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
- Bimolecular Fluorescence Complementation as a Tool to Study Specific Dynamic Interactions Between Proteins in Fission Yeast.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Metaphase kinetochore movements are regulated by kinesin-8 motors and microtubule dynamic instability.Molecular biology of the cell · 2018Article
- Phosphorylation of the RNA-binding protein Zfs1 modulates sexual differentiation in fission yeast.Journal of cell science · 2017Article
- Paired arrangement of kinetochores together with microtubule pivoting and dynamics drive kinetochore capture in meiosis I.Scientific reports · 2016Article
- Regulating retrotransposon activity through the use of alternative transcription start sites.EMBO reports · 2016Article
- A CRISPR/Cas9-based method and primer design tool for seamless genome editing in fission yeast.Wellcome open research · 2016Article
- Article
- Pivoting of microtubules around the spindle pole accelerates kinetochore capture.Nature cell biology · 2013Article
- An improved strategy for tandem affinity purification-tagging of Schizosaccharomyces pombe genes.Proteomics · 2009Article
- Sites of strong Rec12/Spo11 binding in the fission yeast genome are associated with meiotic recombination and with centromeres.Chromosoma · 2008Article
- urg1: a uracil-regulatable promoter system for fission yeast with short induction and repression times.PloS one · 2008Article
- High-throughput knockout screen in fission yeast.Nature protocols · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
PCR-based gene targeting is a popular method for manipulating yeast genes in their normal chromosomal locations. The manual design of primers, however, can be cumbersome and error-prone. We have developed a straightforward web-based tool that applies user-specified inputs to automate and simplify the task of primer selection for deletion, tagging and/or regulated expression of genes in Schizosaccharomyces pombe. This tool, named PPPP (for Pombe PCR Primer Programs), is available at http://www.sanger.ac.uk/PostGenomics/S_pombe/software/. We also present a searchable Microarray Primer Database to retrieve the sequences and accompanying information for primers and PCR products used to build our in-house Sz. pombe microarrays. This database contains information on both coding and intergenic regions to provide context for the microarray data, and it should be useful also for other applications, such as quantitative PCR. The database can be accessed at http://www.sanger.ac.uk/PostGenomics/S_pombe/microarray/.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.