ArticleTransgenic research2006
Generation of marker- and backbone-free transgenic potatoes by site-specific recombination and a bi-functional marker gene in a non-regular one-border agrobacterium transformation vector.
Article in Transgenic research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Targeted mutagenesis with sequence-specific nucleases for accelerated improvement of polyploid crops: Progress, challenges, and prospects.The plant genome · 2023Review
- Multi-allelic gene editing in an apomictic, tetraploid turf and forage grass (Frontiers in plant science · 2023Article
- Article
- State of the Art of Genetic Engineering in Potato: From the First Report to Its Future Potential.Frontiers in plant science · 2021Review
- Generation of transgene-free PDS mutants in potato by Agrobacterium-mediated transformation.BMC biotechnology · 2020Article
- Coexpression of octopine and succinamopine Agrobacterium virulence genes to generate high quality transgenic events in maize by reducing vector backbone integration.Transgenic research · 2018Article
- An Insight into T-DNA Integration Events in Medicago sativa.International journal of molecular sciences · 2017Article
- Intein-mediated Cre protein assembly for transgene excision in hybrid progeny of transgenic Arabidopsis.Plant cell reports · 2016Article
- Screening for recombinants of Crambe abyssynica after transformation by the pMF1 marker-free vector based on chemical selection and meristematic regeneration.Scientific reports · 2015Article
- Large-scale production and evaluation of marker-freeMolecular breeding : new strategies in plant improvement · 2014Article
- Maize transformation technology development for commercial event generation.Frontiers in plant science · 2014Review
- Less is more: strategies to remove marker genes from transgenic plants.BMC biotechnology · 2013Review
- Recent advances in development of marker-free transgenic plants: regulation and biosafety concern.Journal of biosciences · 2012Review
- Improved FLP recombinase, FLPe, efficiently removes marker gene from transgene locus developed by Cre-lox mediated site-specific gene integration in rice.Molecular biotechnology · 2011Article
- Recombinase technology: applications and possibilities.Plant cell reports · 2011Review
- A novel mannose-based selection system for plant transformation using celery mannose-6-phosphate reductase gene.Plant cell reports · 2010Article
- Article
- Production of transgenic barrel medic (Medicago truncatula Gaernt.) using the ipt-type MAT vector system and impairment of Recombinase-mediated excision events.Plant cell reports · 2009Article
- Cytokinin vectors mediate marker-free and backbone-free plant transformation.Transgenic research · 2008Article
- Obtaining marker-free transgenic plants.Doklady. Biochemistry and biophysicsArticle
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A binary vector, designated PROGMO, was constructed to assess the potential of the Zygosaccharomyces rouxii R/Rs recombination system for generating marker- and backbone-free transgenic potato (Solanum tuberosum) plants with high transgene expression and low copy number insertion. The PROGMO vector utilises a constitutively expressed plant-adapted R recombinase and a codA-nptII bi-functional, positive/negative selectable marker gene. It carries only the right border (RB) of T-DNA and consequently the whole plasmid will be inserted as one long T-DNA into the plant genome. The recognition sites (Rs) are located at such positions that recombinase enzyme activity will recombine and delete both the bi-functional marker genes as well as the backbone of the binary vector, leaving only the gene of interest flanked by a copy of Rs and RB. Efficiency of PROGMO transformation was tested by introduction of the GUS reporter gene into potato. It was shown that after 21 days of positive selection and using 300 mgl(-1 )5-fluorocytosine for negative selection, 29% of regenerated shoots carried only the GUS gene flanked by a copy of Rs and RB. The PROGMO vector approach is simple and might be widely applicable for the production of marker- and backbone-free transgenic plants of many crop species.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.