ArticleJournal of bacteriology2007
The RpoT regulon of Pseudomonas putida DOT-T1E and its role in stress endurance against solvents.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- CsbD, a Novel Group B Streptococcal Stress Response Factor That Contributes to Bacterial Resistance against Environmental Bile Salts.Journal of bacteriology · 2023Article
- A mutation in the AdhE alcohol dehydrogenase of Clostridium thermocellum increases tolerance to several primary alcohols, including isobutanol, n-butanol and ethanol.Scientific reports · 2019Article
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- Engineering Biological Approaches for Detection of Toxic Compounds: A New Microbial Biosensor Based on the Pseudomonas putida TtgR Repressor.Molecular biotechnology · 2015Article
- Knockout of extracytoplasmic function sigma factor ECF-10 affects stress resistance and biofilm formation in Pseudomonas putida KT2440.Applied and environmental microbiology · 2014Article
- Characterization of five ECF sigma factors in the genome of Pseudomonas syringae pv. syringae B728a.PloS one · 2013Article
- Enhanced tolerance to naphthalene and enhanced rhizoremediation performance for Pseudomonas putida KT2440 via the NAH7 catabolic plasmid.Applied and environmental microbiology · 2012Article
- Mechanisms of resistance to chloramphenicol in Pseudomonas putida KT2440.Antimicrobial agents and chemotherapy · 2012Article
- Microbial responses to xenobiotic compounds. Identification of genes that allow Pseudomonas putida KT2440 to cope with 2,4,6-trinitrotoluene.Microbial biotechnology · 2009Article
- A two-component regulatory system integrates redox state and population density sensing in Pseudomonas putida.Journal of bacteriology · 2008Article
- A set of activators and repressors control peripheral glucose pathways in Pseudomonas putida to yield a common central intermediate.Journal of bacteriology · 2008Article
- Physiological responses of Pseudomonas putida to formaldehyde during detoxification.Microbial biotechnology · 2008Article
- The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses.Molecular microbiology · 2008Article
- Convergent peripheral pathways catalyze initial glucose catabolism in Pseudomonas putida: genomic and flux analysis.Journal of bacteriology · 2007Article
- Genomotyping of Pseudomonas putida strains using P. putida KT2440-based high-density DNA microarrays: implications for transcriptomics studies.Applied microbiology and biotechnology · 2007Article
- Metabolic analysis of the response ofMetabolomics : Official journal of the Metabolomic SocietyArticle
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas putida encodes 20 extracytoplasmic sigma factors (ECFs). In this study, we show that one of these ECFs, known as ECF-Pp12 (PP3006), plays a role in tolerance of toluene and other organic solvents. Based on this finding, we have called the gene that encodes this new ECF rpoT. The rpoT gene forms an operon with the preceding gene and with the gene located downstream. The translated gene product of the open reading frame PP3005 is an inner membrane protein, whereas the PP3007 protein is periplasmic. A nonpolar DeltarpoT mutant was generated by homologous recombination, and survival of the mutant was tested under various stress conditions. The mutant strain was hypersensitive to toluene and other solvents but just as tolerant as the wild type of stress imposed by heat, antibiotics, NaCl, paraquat, sodium dodecyl sulfate, H(2)O(2), and benzoate. In the DeltarpoT mutant background, expression of around 50 transcriptional units was affected: 31 cistrons were upregulated, and 23 cistrons were downregulated. This indicates that about 1% of all P. putida genes are under the direct or indirect influence of RpoT. The rpoT gene controls the expression of a number of membrane proteins, including components of the respiratory chains, porins, transporters, and multidrug efflux pumps. Hypersensitivity of the P. putida RpoT-deficient mutant to organic solvents can be attributed to the fact that in the DeltarpoT strain, expression of the toluene efflux pump ttgGHI genes is severalfold lower than in the parental strain.
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