ArticleProceedings of the National Academy of Sciences of the United States of America2006
CLA-1 and its splicing variant CLA-2 mediate bacterial adhesion and cytosolic bacterial invasion in mammalian cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 74 citations in OpenAlex.
- Different Functions of Human Scavenger Receptors BI and BII Overexpressed in a Murine Abdominal Sepsis Model.Biomolecules · 2026Article
- High-density lipoprotein mediates silica nanoparticle recognition by the multiligand receptor SR-B1.The Journal of biological chemistry · 2026Article
- The potential role of scavenger receptor B type I (SR-BI) in SARS-CoV-2 infection.Immunity, inflammation and disease · 2023Review
- Scavenger receptor B1 facilitates the endocytosis of Escherichia coli via TLR4 signaling in mammary gland infection.Cell communication and signaling : CCS · 2023Article
- Article
- Class B Scavenger Receptors BI and BII Protect against LPS-Induced Acute Lung Injury in Mice by Mediating LPS.Infection and immunity · 2021Article
- Scavenger receptor B type 1: expression, molecular regulation, and cholesterol transport function.Journal of lipid research · 2018Review
- Rare SCARB1 mutations associate with high-density lipoprotein cholesterol but not with coronary artery disease.European heart journal · 2018Article
- A Novel Lipopolysaccharide Recognition Mechanism Mediated by Internalization in Teleost Macrophages.Frontiers in immunology · 2018Article
- Significant Down-Regulation of "Biological Adhesion" Genes in Porcine Oocytes after IVM.International journal of molecular sciences · 2017Article
- Human SR-BII mediates SAA uptake and contributes to SAA pro-inflammatory signaling in vitro and in vivo.PloS one · 2017Article
- Distinct surveillance pathway for immunopathology during acute infection via autophagy and SR-BI.Scientific reports · 2016Article
- Human SR-BI and SR-BII Potentiate Lipopolysaccharide-Induced Inflammation and Acute Liver and Kidney Injury in Mice.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Formation of a protein corona on silver nanoparticles mediates cellular toxicity via scavenger receptors.Toxicological sciences : an official journal of the Society of Toxicology · 2015Article
- Hepatic scavenger receptor BI protects against polymicrobial-induced sepsis through promoting LPS clearance in mice.The Journal of biological chemistry · 2014Article
- Scavenger receptor class B type I and immune dysfunctions.Current opinion in endocrinology, diabetes, and obesity · 2014Review
- Artesunate reduces serum lipopolysaccharide in cecal ligation/puncture mice via enhanced LPS internalization by macrophages through increased mRNA expression of scavenger receptors.International journal of molecular sciences · 2014Article
- Macrophage SR-BI regulates LPS-induced pro-inflammatory signaling in mice and isolated macrophages.Journal of lipid research · 2012Article
- Crosstalk between reverse cholesterol transport and innate immunity.Trends in endocrinology and metabolism: TEM · 2012Review
- Class B scavenger receptor types I and II and CD36 targeting improves sepsis survival and acute outcomes in mice.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
Corrections and comments
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Authors and funding
13 authors at 5 institutions in 2 countries.
Funding
Abstract
CD36 and LIMPII analog 1, CLA-1, and its splicing variant, CLA-2 (SR-BI and SR-BII in rodents), are human high density lipoprotein receptors with an identical extracellular domain which binds a spectrum of ligands including bacterial cell wall components. In this study, CLA-1- and CLA-2-stably transfected HeLa and HEK293 cells demonstrated several-fold increases in the uptake of various bacteria over mock-transfected cells. All bacteria tested, including both Gram-negatives (Escherichia coli K12, K1 and Salmonella typhimurium) and Gram-positives (Staphylococcus aureus and Listeria monocytogenes), demonstrated various degrees of lower uptake in control cells. This result is consistent with the presence of high-density lipoprotein-receptor-independent bacterial uptake that is enhanced by CLA-1/CLA-2 overexpression. Bacterial lipopolysaccharides, lipoteichoic acid, and synthetic amphipathic helical peptides (L-37pA and D-37pA) competed with E. coli K12 for CLA-1 and CLA-2 binding. Transmission electron microscopy and confocal microscopy revealed cytosolic accumulation of bacteria in CLA-1/CLA-2-overexpressing HeLa cells. The antibiotic protection assay confirmed that E. coli K12 was able to survive and replicate intracellularly in CLA-1- and CLA-2-overexpressing HeLa, but both L-37pA and D-37pA prevented E. coli K12 invasion. Peritoneal macrophages isolated from SR-BI/BII-knockout mice demonstrated a 30% decrease in bacterial uptake when compared with macrophages from normal mice. Knockout macrophages were also characterized by decreased bacterial cytosolic invasion, ubiquitination, and proteasome mobilization while retaining bacterial lysosomal accumulation. These results indicate that, by facilitating bacterial adhesion and cytosolic invasion, CLA-1 and CLA-2 may play an important role in infection and sepsis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.