ArticlePflugers Archiv : European journal of physiology1991
Steep concentration dependence and fast desensitization of nicotinic channel currents elicited by acetylcholine pulses, studied in adult vertebrate muscle.
Article in Pflugers Archiv : European journal of physiology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 37 citations in OpenAlex.
- Article
- Long-term desensitization of nicotinic acetylcholine receptors is regulated via protein kinase A-mediated phosphorylation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Open channel and competitive block of the embryonic form of the nicotinic receptor of mouse myotubes by (+)-tubocurarine.The Journal of physiology · 1996Article
- Acetylcholine-induced currents in acutely dissociated sympathetic neurons from adult hypertensive and normotensive rats have similar properties.Pflugers Archiv : European journal of physiology · 1995Article
- Gating kinetics of the quisqualate-sensitive glutamate receptor of locust muscle studied using agonist concentration jumps and computer simulations.Biophysical journal · 1993Article
- A molecular scheme for the reaction between acetylcholine and nicotinic channels.Biophysical journal · 1993Article
- Contribution of Ca2+ inflow to quantal, phasic transmitter release from nerve terminals of frog muscle.Pflugers Archiv : European journal of physiology · 1992Article
- A study of the bovine adrenal chromaffin nicotinic receptor using patch clamp and concentration-jump techniques.The Journal of physiology · 1992Article
- Activation and desensitization of N-methyl-D-aspartate receptors in nucleated outside-out patches from mouse neurones.The Journal of physiology · 1992Article
- Desensitization of acetylcholine receptors in BC3H-1 cells.Pflugers Archiv : European journal of physiology · 1992Article
- Alpha 4-2 beta 2 and other nicotinic acetylcholine receptor subtypes as targets of psychoactive and addictive drugs.British journal of pharmacology · 1992Article
- Activation and desensitization of embryonic-like receptor channels in mouse muscle by acetylcholine concentration steps.The Journal of physiology · 1992Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Skeletal muscles of adult mice and frogs were dissociated enzymatically and prepared for patch-clamping within less than 6 h. Outside-out patches were superfused with repetitive pulses of acetylcholine (ACh) with switching times of about 0.2 ms. Peak responses were reached within 1 ms. In mouse muscle the average channel conductance was 65 pS and the average open time 1 ms (20 degrees C). Between 1 and 10 microM ACh, the peak responses increased proportional to the second to third power of the ACh concentration, and less steeply between 10 and 1000 microM ACh. The apparent Km of the dose-response curve was about 100 microM. After the peak, channel opening probability declined with time constants decreasing from about 1 s with 1 microM ACh to 15-50 ms with 1 mM ACh. After 100 ms desensitization the channel opening had decreased to less than 1/300 peak value. The rate of desensitization increased with rising temperature, with Q10 values of 1.7-2.5 between 10 and 30 degrees C. The desensitization characteristics of channels from frog muscle were similar to that from mice. With pulses of 100 microM ACh the channels opened with a probability of 0.55, the open probability declining with a time constant of about 60 ms and dropping to less than 0.001 after 300 ms. The results support the view that three binding steps of ACh are necessary for opening of the channel. Desensitization in the presence of high ACh concentrations is slower than the decay of synaptic currents.
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