ArticleHistochemistry1990
Contrasting of Lowicryl K4M thin sections.
Article in Histochemistry, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 89 citations in OpenAlex.
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- Intracellular membranes of bacterial endospores are reservoirs for spore core membrane expansion during spore germination.Scientific reports · 2018Article
- Molecular in situ topology of Aczonin/Piccolo and associated proteins at the mammalian neurotransmitter release site.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Functional characterization of Negri bodies (NBs) in rabies virus-infected cells: Evidence that NBs are sites of viral transcription and replication.Journal of virology · 2009Article
- Co-Localization of beta-1,3-Glucanases and Callose During Somatic Embryogenesis in Cichorium.Plant signaling & behavior · 2007Article
- Rapid diagnostic thin section electron microscopy of bacterial endospores.Journal of microbiological methods · 2007Article
- Preferential localization of rat GAPDS on the ribs of fibrous sheath of sperm flagellum and its expression during flagellar formation.Acta histochemica et cytochemica · 2007Article
- A mutation in the insulin 2 gene induces diabetes with severe pancreatic beta-cell dysfunction in the Mody mouse.The Journal of clinical investigation · 1999Article
- The human T-cell leukemia virus type 1 transactivator protein Tax colocalizes in unique nuclear structures with NF-kappaB proteins.Journal of virology · 1997Article
- Protein glycosylation in the endoplasmic reticulum and the Golgi apparatus and cell type-specificity of cell surface glycoconjugate expression: analysis by the protein A-gold and lectin-gold techniques.Histochemistry and cell biology · 1996Review
- The silver anniversary of gold: 25 years of the colloidal gold marker system for immunocytochemistry and histochemistry.Histochemistry and cell biology · 1996Review
- Topographic abnormalities of proinsulin to insulin conversion in functioning human insulinomas. Comparison of immunoelectron microscopic and clinical data.The American journal of pathology · 1995Article
- Subcellular distribution of terminal alpha-D- and beta-D-galactosyl residues in Ehrlich tumour cells studied by lectin-gold techniques.Glycoconjugate journal · 1995Article
- Postembedding alpha-tubulin immunolabelling of isolated centrosomes.The Histochemical journal · 1995Article
- Cytochemical staining for beta 1,6 branching of asparagine-linked oligosaccharides in variants of metastatic human colon carcinoma cells.The American journal of pathology · 1994Article
- Article
- Alpha IIb beta 3 integrin dissociation induced by EDTA results in morphological changes of the platelet surface-connected canalicular system with differential location of the two separate subunits.The Journal of cell biology · 1993Article
- Characterization of the antigen recognized by a monoclonal antibody MN9: unique transport pathway to the equatorial segment of sperm head during spermiogenesis.Cell and tissue research · 1992Article
- Specific binding of human fibrinogen fragment D to Aspergillus fumigatus conidia.Infection and immunity · 1992Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
A method is presented for increasing the contrast of cellular structures on ultrathin sections from tissues embedded in Lowicryl K4M. The method, designated UA/MC adsorption staining, is based on the uranyl acetate/methyl cellulose staining of thawed cryosections. Ultrathin Lowicryl K4M sections were exposed to a uranyl acetate/methyl cellulose solution and the excess solution was removed with filter paper, leaving the remainder to air dry on the section. Sections on the grids were then directly observed in the electron microscope. Parameters such as methyl cellulose and uranyl acetate concentrations, duration of staining, temperature and pH were all assessed for their effect on subsequent contrast formation. Conditions were achieved which yielded intense contrast of cellular membranes, basement membranes and extracellular matrix components usually not apparent in Lowicryl K4M thin sections routinely counter-stained with uranyl acetate and lead acetate. The enhancement of the contrast of these structures does not obscure colloidal gold particles used for immunocytochemistry or lectin labeling, thus making the UA/MC adsorption staining method useful for increasing membrane contrast in routine post-embedding immuno- and lectin cytochemistry on Lowicryl K4M thin sections.
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