ArticleCarbohydrate research2007
Synthesis of reference standards to enable single cell metabolomic studies of tetramethylrhodamine-labeled ganglioside GM1.
Article in Carbohydrate research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 34 citations in OpenAlex.
- Raft-based interactions of gangliosides with a GPI-anchored receptor.Nature chemical biology · 2016Article
- A facile method for controlling the reaction equilibrium of sphingolipid ceramide N-deacylase for lyso-glycosphingolipid production.Journal of lipid research · 2015Article
- Partial synthesis of ganglioside and lysoganglioside lipoforms as internal standards for MS quantification.Journal of lipid research · 2014Article
- Single cell metabolic profiling of tumor mimics.Analytical chemistry · 2013Article
- Capillary electrophoresis with three-color fluorescence detection for the analysis of glycosphingolipid metabolism.The Analyst · 2013Article
- Thiogalactopyranosides are resistant to hydrolysis by α-galactosidases.Chembiochem : a European journal of chemical biology · 2012Article
- Single cell ganglioside catabolism in primary cerebellar neurons and glia.Neurochemical research · 2012Article
- Metabolic cytometry: capillary electrophoresis with two-color fluorescence detection for the simultaneous study of two glycosphingolipid metabolic pathways in single primary neurons.Analytical chemistry · 2012Article
- Preparation and electrophoretic separation of Bodipy-Fl-labeled glycosphingolipids.Journal of chromatography. A · 2012Article
- PmST2: a novel Pasteurella multocida glycolipid α2-3-sialyltransferase.Glycobiology · 2011Article
- Nine orders of magnitude dynamic range: picomolar to millimolar concentration measurement in capillary electrophoresis with laser induced fluorescence detection employing cascaded avalanche photodiode photon counters.Analytical chemistry · 2011Article
- Monitoring the uptake of glycosphingolipids in Plasmodium falciparum-infected erythrocytes using both fluorescence microscopy and capillary electrophoresis with laser-induced fluorescence detection.Analytical chemistry · 2010Article
- Indium-tin oxide coated microfabricated device for the injection of a single cell into a fused silica capillary for chemical cytometry.Analytical chemistry · 2010Article
- Capillary array isoelectric focusing with laser-induced fluorescence detection: milli-pH unit resolution and yoctomole mass detection limits in a 32-channel system.Analytical and bioanalytical chemistry · 2010Article
- Interface of an array of five capillaries with an array of one-nanoliter wells for high-resolution electrophoretic analysis as an approach to high-throughput chemical cytometry.Analytical chemistry · 2008Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
Ganglioside GM1 and its seven potential catabolic products: asialo-GM1, GM2, asialo-GM2, GM3, Lac-Cer, Glc-Cer and Cer, were labeled with tetramethylrhodamine (TMR) to permit ultra-sensitive analysis using laser-induced fluorescence (LIF) detection. The preparation involved acylation of the homogenous C(18)lyso-forms of GM1, Lac-Cer, Glc-Cer and Cer with the N-hydroxysuccinimide ester of a beta-alanine-tethered 6-TMR derivative, followed by conversion of these labeled products using galactosidase, sialidase, and sialyltransferase enzymes. The TMR-glycolipid analogs produced are detectable on TLC down to the 1 ng level by the naked eye. All eight compounds could be separated within 4 min in capillary electrophoresis where they could be detected at the zeptomole (ca. 1000 molecule) level using LIF.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.