ArticlePflugers Archiv : European journal of physiology1990
Calcium currents in the A7r5 smooth muscle-derived cell line.
Article in Pflugers Archiv : European journal of physiology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 99 citations in OpenAlex.
- Role and mechanism of G9A-BMP2 in calcification-induced formation of unstable plaque.Atherosclerosis plus · 2026Article
- 4-Pyridinio-1,4-Dihydropyridines as Calcium Ion Transport Modulators: Antagonist, Agonist, and Dual Action.Oxidative medicine and cellular longevity · 2020Article
- Heme oxygenase-1 regulates cell proliferation via carbon monoxide-mediated inhibition of T-type Ca2+ channels.Pflugers Archiv : European journal of physiology · 2015Article
- A naturally occurring truncated Cav1.2 α1-subunit inhibits Ca2+ current in A7r5 cells.American journal of physiology. Cell physiology · 2013Article
- Regulation of the cardiac L-type calcium channel in L6 cells by arginine-vasopressin.The Biochemical journal · 2006Article
- Ca2+ signalling in rat vascular smooth muscle cells: a role for protein kinase C at physiological vasoconstrictor concentrations of vasopressin.The Journal of physiology · 2000Article
- Multiple channels mediate calcium leakage in the A7r5 smooth muscle-derived cell line.Biophysical journal · 1998Article
- Regulation of the L-type calcium channel alpha-1 subunit by chronic depolarization in the neuron-like PC12 and aortic smooth muscle A7r5 cell lines.Pflugers Archiv : European journal of physiology · 1995Article
- Evidence for a direct interaction of thapsigargin with voltage-dependent Ca2+ channel.Naunyn-Schmiedeberg's archives of pharmacology · 1995Article
- Differential beta-adrenergic regulation and phenotypic modulation of voltage-gated calcium currents in rat aortic myocytes.The Journal of physiology · 1994Article
- Isoproterenol modulates the calcium channels through two different mechanisms in smooth-muscle cells from rabbit portal vein.Pflugers Archiv : European journal of physiology · 1994Article
- Protein kinase A increases availability of calcium channels in smooth muscle cells from guinea pig basilar artery.Pflugers Archiv : European journal of physiology · 1994Article
- Effect of isoprenaline on Ca2+ channel current in single smooth muscle cells isolated from taenia of the guinea-pig caecum.The Journal of physiology · 1993Article
- Calcium currents in the A7r5 smooth muscle-derived cell line. An allosteric model for calcium channel activation and dihydropyridine agonist action.The Journal of general physiology · 1992Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have studied voltage-dependent calcium channels in the A7r5 smooth muscle cell line by measuring the high-affinity binding of radiolabelled dihydropyridines (DHPs), whole-cell and single-channel currents in patch-clamped cells, as well as cytosolic calcium ([Ca2+]i) in fura-2-loaded cell suspensions and monolayers. Intact A7r5 cells express saturable, high-affinity, voltage-sensitive DHP binding sites with pharmacological properties characteristic of L-type calcium channels. When cells were voltage clamped in the whole-cell configuration with near normal intra- and extracellular solutions, a DHP-sensitive inward current resembling the L-type calcium current was dominant. With barium (10 mM) as the charge carrier, peak inward currents were typically recorded at test potentials between 0 and +20 mV. Currents were blocked by extracellular cadmium with a half-maximal inhibitory concentration of approximately 1 microM. Isoproterenol (1 microM) or forskolin (10 microM) increased currents in approximately half of the cells tested. Forskolin (10 microM) increased single-channel activity in five of eight cell-attached patches. After cells had been quiescent for several weeks, cell suspensions showed changes in resting [Ca2+]i in response to DHPs and increased potassium. Most confluent monolayers of cells showed spontaneous transient elevations in [Ca2+]i. Bath application of Bay K 8644 increased the frequency and magnitude of these [Ca2+]i transients, whereas nifedipine abolished the transients. These data suggest that the [Ca2+]i transients were due to synchronous action potentials in electrically coupled cell monolayers.
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