ArticleRNA (New York, N.Y.)2006
Imaging the alternative silencing of FGFR2 exon IIIb in vivo.
Article in RNA (New York, N.Y.), 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Acne Syndromes and Mosaicism.Biomedicines · 2021Review
- The VEGF-A exon 8 splicing-sensitive fluorescent reporter mouse is a novel tool to assess the effects of splicing regulatory compoundsRNA biology · 2019Article
- Pharmacology of Modulators of Alternative Splicing.Pharmacological reviews · 2017Review
- Fluorescence-based alternative splicing reporters for the study of epithelial plasticity in vivo.RNA (New York, N.Y.) · 2013Article
- Alternative Splicing of Fibroblast Growth Factor Receptor IgIII Loops in Cancer.Journal of nucleic acids · 2012Article
- Efficient in vivo manipulation of alternative pre-mRNA splicing events using antisense morpholinos in mice.The Journal of biological chemistry · 2011Article
- Visualization and genetic analysis of alternative splicing regulation in vivo using fluorescence reporters in transgenic Caenorhabditis elegans.Nature protocols · 2010Article
- Splicing reporter mice revealed the evolutionally conserved switching mechanism of tissue-specific alternative exon selection.PloS one · 2010Article
- Fox-1 family of RNA-binding proteins.Cellular and molecular life sciences : CMLS · 2009Review
- Identification of an intronic splicing enhancer essential for the inclusion of FGFR2 exon IIIc.The Journal of biological chemistry · 2008Article
- Noninvasive molecular neuroimaging using reporter genes: part II, experimental, current, and future applications.AJNR. American journal of neuroradiology · 2008Review
- STAR family RNA-binding protein ASD-2 regulates developmental switching of mutually exclusive alternative splicing in vivo.Genes & development · 2008Article
- Dunning rat prostate adenocarcinomas and alternative splicing reporters: powerful tools to study epithelial plasticity in prostate tumors in vivo.Clinical & experimental metastasis · 2008Article
- The impact of alternative splicing in vivo: mouse models show the way.RNA (New York, N.Y.) · 2007Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Alternative splicing multiplies genomic coding capacity and regulates proteomic composition. A well-studied example of this plasticity leads to the synthesis of functionally distinct isoforms of the Fibroblast Growth Factor Receptor-2 (FGFR2). The regulation of this isoform diversity necessitates the silencing of FGFR2 exon IIIb, which is mediated by flanking intronic splicing silencers and the polypyrimidine tract binding protein (PTB). To visualize this splicing decision in vivo, we developed mice harboring a green fluorescent protein construct that reports on the silencing of exon IIIb. The animals also harbor a red fluorescent protein reporter of constitutive splicing as an allelic control. This dual reporter system revealed that in various organs and cell types the silencing of exon IIIb required the intronic silencers. In neurons, which do not express PTB, we observed robust silencer-dependent repression of exon IIIb, suggesting that the neural paralog, brain PTB, can take over this function. In the epidermis, however, the intronic silencers were not required for efficient silencing. This work provides a first glimpse at splicing regulation among different cell types in vivo and promises the drafting of an anatomic map of splicing decisions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.