ReviewCell calcium
Imaging single-channel calcium microdomains.
Review in Cell calcium. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 82 citations in OpenAlex.
- Cell type-specific Ca2+ signals govern mouse seminiferous tubule physiology.PLoS biology · 2026Article
- Review
- Bound CaFrontiers in physiology · 2023Article
- Roles and Sources of Calcium in Synaptic Exocytosis.Advances in neurobiology · 2023Article
- Mechanisms and physiological implications of cooperative gating of clustered ion channels.Physiological reviews · 2022Review
- Ion Channels in Glioma Malignancy.Reviews of physiology, biochemistry and pharmacology · 2021Review
- Fundamentals of Cellular Calcium Signaling: A Primer.Cold Spring Harbor perspectives in biology · 2020Review
- Rethinking calcium profiles around single channels: the exponential and periodic calcium nanodomains.Scientific reports · 2019Article
- Spatial-temporal patterning of CaSeminars in cell & developmental biology · 2019Review
- CellSpecks: A Software for Automated Detection and Analysis of Calcium Channels in Live Cells.Biophysical journal · 2018Article
- TraceSpecks: A Software for Automated Idealization of Noisy Patch-Clamp and Imaging Data.Biophysical journal · 2018Article
- Real-Time Sensing of Single-Ligand Delivery with Nanoaperture-Integrated Microfluidic Devices.ACS omega · 2017Article
- CaThe Journal of physiology · 2017Review
- Spinning-Spot Shadowless TIRF Microscopy.PloS one · 2015Article
- Single-molecule patch-clamp FRET microscopy studies of NMDA receptor ion channel dynamics in living cells: revealing the multiple conformational states associated with a channel at its electrical off state.Journal of the American Chemical Society · 2014Article
- The influence of Ca²⁺ buffers on free [Ca²⁺] fluctuations and the effective volume of Ca²⁺ microdomains.Biophysical journal · 2014Article
- Calcium transport and local pool regulate polycystin-2 (TRPP2) function in human syncytiotrophoblast.Biophysical journal · 2013Article
- The Xenopus oocyte: a single-cell model for studying Ca2+ signaling.Cold Spring Harbor protocols · 2013Article
- Actin-based confinement of calcium responses during Shigella invasion.Nature communications · 2013Article
- Optical recording reveals novel properties of GSK1016790A-induced vanilloid transient receptor potential channel TRPV4 activity in primary human endothelial cells.Molecular pharmacology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The Ca(2+) microdomains generated around the mouth of open ion channels represent the basic building blocks from which cytosolic Ca(2+) signals are constructed. Recent improvements in optical imaging techniques now allow these microdomains to be visualized as single channel calcium fluorescence transients (SCCaFTs), providing information about channel properties that was previously accessible only by electrophysiological patch-clamp recordings. We review recent advances in single channel Ca(2+) imaging methodologies, with emphasis on total internal reflection fluorescence microscopy (TIRFM) as the technique of choice for recording SCCaFTs from voltage- and ligand-gated plasmalemmal ion channels. This technique of 'optical patch-clamp recording' is massively parallel, permitting simultaneous imaging of hundreds of channels; provides millisecond resolution of gating kinetics together with sub-micron spatial resolution of channel locations; and is applicable to diverse families of membrane channels that display partial permeability to Ca(2+) ions.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.