ArticleExperimental eye research2007
Examination of the restoration of epithelial barrier function following superficial keratectomy.
Article in Experimental eye research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
- Hepatocyte growth factor upregulates MMP1 and MMP10 expression and resolves corneal fibrosis.Scientific reports · 2024Article
- Comparative study on corneal epithelium healing: effects of crosslinked hyaluronic acid and amniotic membrane extract eye drops in rats.Frontiers in veterinary science · 2024Article
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- The Efficacy of Topical HGF on Corneal Fibrosis and Epithelial Healing after Scar-Producing PRK Injury in Rabbits.Translational vision science & technology · 2020Article
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- Hepatocyte Growth Factor Suppresses Inflammation and Promotes Epithelium Repair in Corneal Injury.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
- Creation of a Bioengineered Skin Flap Scaffold with a Perfusable Vascular Pedicle.Tissue engineering. Part A · 2017Article
- Restoration of Corneal Transparency by Mesenchymal Stem Cells.Stem cell reports · 2016Article
- Novel Technique of Transepithelial Corneal Cross-Linking Using Iontophoresis in Progressive Keratoconus.Journal of ophthalmology · 2016Article
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- Experimental orthotopic transplantation of a tissue-engineered oesophagus in rats.Nature communications · 2014Article
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- αVβ6 integrin promotes corneal wound healing.Investigative ophthalmology & visual science · 2011Article
- Noninvasive intratissue refractive index shaping (IRIS) of the cornea with blue femtosecond laser light.Investigative ophthalmology & visual science · 2011Article
- MMP9 cleavage of the β4 integrin ectodomain leads to recurrent epithelial erosions in mice.Journal of cell science · 2011Article
- High-resolution, noninvasive, two-photon fluorescence measurement of molecular concentrations in corneal tissue.Investigative ophthalmology & visual science · 2011Article
- Spontaneous bacterial keratitis in CD36 knockout mice.Investigative ophthalmology & visual science · 2011Article
- Corneal alternations induced by topical application of benzalkonium chloride in rabbit.PloS one · 2011Article
- Autoradiographic study on the regenerative capability of the epithelium lining the center of the cornea after multiple debridements of its peripheral region.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2010Article
- Adenovirus-driven overexpression of proteinases in organ-cultured normal human corneas leads to diabetic-like changes.Brain research bulletin · 2010Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
The goal of the present study was to determine the rate of restoration of the corneal epithelial barrier following a superficial keratectomy using a functional assay of tight junction integrity. Adult Sprague-Dawley rats were anesthetized and a 3-mm superficial keratectomy was performed. The eyes were allowed to heal from 4 h to 8 weeks and the rate of epithelial wound closure was determined. To examine the restoration of the barrier function, EZ-Link Sulfo-NHS-LC-Biotin (LC-Biotin) was applied to all eyes, experimental and control, for 15 min at the time of sacrifice. This compound does not penetrate through intact tight junctions. Indirect immunofluorescence was performed with anti-laminin, a marker of basement membrane; fluorescein-conjugated streptavidin to detect the biotinylated marker; and anti-occludin and anti-ZO-1, markers of tight junctions. Epithelial wound closure was observed at 36-42 h after wounding. LC-Biotin did not penetrate the intact epithelium. Upon wounding, LC-Biotin penetrated into the stroma subjacent and slightly peripheral to the wound area. This pattern was present from 4-48 h post-wounding. The area of LC-Biotin localization decreased with time and the functional barrier was restored by 72 h. Occludin and ZO-1 were present at all time points. The number of cell layers expressing these proteins appeared to increase at 48 and 72 h. Continuous laminin localization was not observed until at least 7 days after wounding. Barrier function is restored within 1-1.5 days after epithelial wound closure. The loss of barrier function does not extend beyond the edge of the original wound. The restoration of barrier function does not appear to correlate with reassembly of the basement membrane in this model.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.