ArticleBMC genomics2006
Genome-wide mapping of ORC and Mcm2p binding sites on tiling arrays and identification of essential ARS consensus sequences in S. cerevisiae.
Article in BMC genomics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
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84 citing papers in PubMed, 120 citations in OpenAlex.
- Dbf4-dependent kinase finetunes Ino80 function at chromosome replication origins.Nature communications · 2026Article
- Ordered and disordered regions of the Origin Recognition Complex direct differential in vivo binding at distinct motif sequences.Nucleic acids research · 2024Article
- Identification of 1600 replication origins ineLife · 2024Article
- Mechanism of eukaryotic origin unwinding is a dual helicase DNA shearing process.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Where and when to start: Regulating DNA replication origin activity in eukaryotic genomes.Nucleus (Austin, Tex.) · 2023Review
- A genome-wide map of DNA replication at single-molecule resolution in the malaria parasite Plasmodium falciparum.Nucleic acids research · 2023Article
- Article
- Nucleoporins facilitate ORC loading onto chromatin.Cell reports · 2022Article
- Origin recognition complex harbors an intrinsic nucleosome remodeling activity.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Stimulation of adaptive gene amplification by origin firing under replication fork constraint.Nucleic acids research · 2022Article
- Article
- Chromosomal Mcm2-7 distribution and the genome replication program in species from yeast to humans.PLoS genetics · 2021Article
- Conservation of a DNA Replication Motif among Phylogenetically Distant Budding Yeast Species.Genome biology and evolution · 2021Article
- Methylation of histone H3 at lysine 37 by Set1 and Set2 prevents spurious DNA replication.Molecular cell · 2021Article
- The capacity of origins to load MCM establishes replication timing patterns.PLoS genetics · 2021Article
- Replication origin-flanking roadblocks reveal origin-licensing dynamics and altered sequence dependence.The Journal of biological chemistry · 2017Article
- Precise Editing at DNA Replication Forks Enables Multiplex Genome Engineering in Eukaryotes.Cell · 2017Article
- Localization of Cdc7 Protein Kinase During DNA Replication inG3 (Bethesda, Md.) · 2017Article
- Firing of Replication Origins Frees Dbf4-Cdc7 to Target Eco1 for Destruction.Current biology : CB · 2017Article
- A Matter of Scale and Dimensions: Chromatin of Chromosome Landmarks in the Fungi.Microbiology spectrum · 2017Review
24 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundEukaryotic replication origins exhibit different initiation efficiencies and activation times within S-phase. Although local chromatin structure and function influences origin activity, the exact mechanisms remain poorly understood. A key to understanding the exact features of chromatin that impinge on replication origin function is to define the precise locations of the DNA sequences that control origin function. In S. cerevisiae, Autonomously Replicating Sequences (ARSs) contain a consensus sequence (ACS) that binds the Origin Recognition Complex (ORC) and is essential for origin function. However, an ACS is not sufficient for origin function and the majority of ACS matches do not function as ORC binding sites, complicating the specific identification of these sites.
resultsTo identify essential origin sequences genome-wide, we utilized a tiled oligonucleotide array (NimbleGen) to map the ORC and Mcm2p binding sites at high resolution. These binding sites define a set of potential Autonomously Replicating Sequences (ARSs), which we term nimARSs. The nimARS set comprises 529 ORC and/or Mcm2p binding sites, which includes 95% of known ARSs, and experimental verification demonstrates that 94% are functional. The resolution of the analysis facilitated identification of potential ACSs (nimACSs) within 370 nimARSs. Cross-validation shows that the nimACS predictions include 58% of known ACSs, and experimental verification indicates that 82% are essential for ARS activity.
conclusionThese findings provide the most comprehensive, accurate, and detailed mapping of ORC binding sites to date, adding to the emerging picture of the chromatin organization of the budding yeast genome.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.