ArticleThe Biochemical journal2007
Structural and biochemical characterization of human orphan DHRS10 reveals a novel cytosolic enzyme with steroid dehydrogenase activity.
Article in The Biochemical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 104 citations in OpenAlex.
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- Single cell resolution of neurosteroidogenesis in the murine brain: intermediary biosynthesis.The Journal of endocrinology · 2025Article
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- Glial expression of a steroidogenic enzyme underlies natural variation in hitchhiking behavior.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Inhibiting HSD17B8 suppresses the cell proliferation caused by PTEN failure.Scientific reports · 2024Article
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- Dynamic regulation of the transcriptome and proteome of the equine embryo during maternal recognition of pregnancy.FASEB bioAdvances · 2022Article
- Model Cell Lines and Tissues of Different HGSOC Subtypes Differ in Local Estrogen Biosynthesis.Cancers · 2022Article
- Analogues of Natural Chalcones as Efficient Inhibitors of AKR1C3.Metabolites · 2022Article
- Association of Coding Variants in Hydroxysteroid 17-beta Dehydrogenase 14 (Journal of the American Society of Nephrology : JASN · 2021Article
- Identification and Characterization of lncRNA and mRNA in Testes of Landrace and Hezuo Boars.Animals : an open access journal from MDPI · 2021Article
- Identification of Functional Single Nucleotide Polymorphisms in PorcineBiomolecules · 2020Article
- Phylogenetics-based identification and characterization of a superior 2,3-butanediol dehydrogenase for Zymomonas mobilis expression.Biotechnology for biofuels · 2020Article
- Short-Chain Dehydrogenase NcmD Is Responsible for the C-10 Oxidation of Nocamycin F in Nocamycin Biosynthesis.Frontiers in microbiology · 2020Article
Corrections and comments
- Erratum issuedKavanagh, Kathryn [Kavanagh, Kathryn L]; Hwa, Lee Wen [corrected to Lee, Wen Hwa]
Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
To this day, a significant proportion of the human genome remains devoid of functional characterization. In this study, we present evidence that the previously functionally uncharacterized product of the human DHRS10 gene is endowed with 17beta-HSD (17beta-hydroxysteroid dehydrogenase) activity. 17beta-HSD enzymes are primarily involved in the metabolism of steroids at the C-17 position and also of other substrates such as fatty acids, prostaglandins and xenobiotics. In vitro, DHRS10 converts NAD+ into NADH in the presence of oestradiol, testosterone and 5-androstene-3beta,17beta-diol. Furthermore, the product of oestradiol oxidation, oestrone, was identified in intact cells transfected with a construct plasmid encoding the DHRS10 protein. In situ fluorescence hybridization studies have revealed the cytoplasmic localization of DHRS10. Along with tissue expression data, this suggests a role for DHRS10 in the local inactivation of steroids in the central nervous system and placenta. The crystal structure of the DHRS10 apoenzyme exhibits secondary structure of the SDR (short-chain dehydrogenase/reductase) family: a Rossmann-fold with variable loops surrounding the active site. It also reveals a broad and deep active site cleft into which NAD+ and oestradiol can be docked in a catalytically competent orientation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.