ArticleMolecular biology of the cell2007
Plasma membrane sterol distribution resembles the surface topography of living cells.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 66 citations in OpenAlex.
- Cell membrane cholesterol affects serotonin transporter efflux due to altered transporter oligomerization.Molecular psychiatry · 2026Article
- Time-Resolved Laurdan Fluorescence Reveals Insights into Membrane Viscosity and Hydration Levels.Biophysical journal · 2018Article
- Cholesterol binding to a conserved site modulates the conformation, pharmacology, and transport kinetics of the human serotonin transporter.The Journal of biological chemistry · 2018Article
- Sphingolipid Organization in the Plasma Membrane and the Mechanisms That Influence It.Frontiers in cell and developmental biology · 2016Review
- Cholesterol segregates into submicrometric domains at the living erythrocyte membrane: evidence and regulation.Cellular and molecular life sciences : CMLS · 2015Article
- Tunneling nanotubes between rat primary astrocytes and C6 glioma cells alter proliferation potential of glioma cells.Neuroscience bulletin · 2015Article
- Fluorescent Sterols and Cholesteryl Esters as Probes for Intracellular Cholesterol Transport.Lipid insights · 2015Review
- Membrane orientation and lateral diffusion of BODIPY-cholesterol as a function of probe structure.Biophysical journal · 2013Article
- Plasma membrane organization and function: moving past lipid rafts.Molecular biology of the cell · 2013Article
- Tunneling-nanotube direction determination in neurons and astrocytes.Cell death & disease · 2012Article
- Analysis of cholesterol trafficking with fluorescent probes.Methods in cell biology · 2012Article
- Tunneling-nanotube development in astrocytes depends on p53 activation.Cell death and differentiation · 2011Article
- Intracellular sterol dynamics.Biochimica et biophysica acta · 2009Review
- Membrane fluidity and lipid order in ternary giant unilamellar vesicles using a new bodipy-cholesterol derivative.Biophysical journal · 2009Article
- Sterols are mainly in the cytoplasmic leaflet of the plasma membrane and the endocytic recycling compartment in CHO cells.Molecular biology of the cell · 2009Article
- Baseline mechanical characterization of J774 macrophages.Biophysical journal · 2009Article
- Review
- Structure of dehydroergosterol monohydrate and interaction with sterol carrier protein-2.Lipids · 2008Article
- Spatiotemporal analysis of endocytosis and membrane distribution of fluorescent sterols in living cells.Histochemistry and cell biology · 2008Article
- TGFbeta as a potential mediator of progesterone action in the mammary gland of pregnancy.Journal of mammary gland biology and neoplasia · 2007Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Cholesterol is an important constituent of cellular membranes. It has been suggested that cholesterol segregates into sterol-rich and -poor domains in the plasma membrane, although clear evidence for this is lacking. By fluorescence imaging of the natural sterol dehydroergosterol (DHE), the lateral sterol distribution has been visualized in living cells. The spatial labeling pattern of DHE coincided with surface structures such as ruffles, microvilli, and filopodia with correlation lengths in the range of 0.8-2.5 microm. DHE staining of branched tubules and of nanotubes connecting two cells was detected. Dynamics of DHE in folded and plane membrane regions was comparable as determined by fluorescence recovery after photobleaching. DHE colocalized with fluid membrane-preferring phospholipids in surface structures and at sites of cell attachment as well as in the cleavage furrow of dividing cells, but it was not particularly enriched in those regions. Fluorescent sterol showed homogeneous staining in membrane blebs induced by F-actin disruption. Cross-linking the ganglioside GM1--a putative raft marker--did not affect the cell surface distribution of DHE. The results suggest that spatial heterogeneities of plasma membrane staining of DHE resolvable by light microscopy reflect the cell surface topography but not phase-separated sterol domains in the bilayer plane.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.